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- W104470612 abstract "The pir locus of R6K encodes a protein (π) that is essential for the initiation of R6K DNA replication. The promoter and coding sequences of this locus were separated and cloned into different plasmids. These various plasmid constructs were used to study the role of π both in regulating the initiation of R6K DNA replication and in regulating its own expression. The π protein was found to reduce levels of β-galactosidase expression from the lacZ gene fused to the pir promoter. In addition, a relatively small change in the cellular concentration of π is observed in response to a 20-fold increase in pir gene dosage. These findings suggest that the expression of the π protein is regulated autogeneously. By fusing the π coding sequences to the tryptophan and pBR322 promoters, the π concentration in vivo could be varied over a wide range. The copy number of an R6K origin plasmid remained essentially the same over a broad range of π concentration, indicating that, although the π protein is required for the initiation of R6K DNA replication, it does not regulate the frequency of this event." @default.
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- W104470612 date "1982-10-01" @default.
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- W104470612 title "Plasmid R6K DNA replication" @default.
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- W104470612 doi "https://doi.org/10.1016/0022-2836(82)90278-9" @default.
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