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- W1044882772 abstract "This chapter discusses the methods for radiolabeling galanin with 125I. Kinetic studies are carried out under pseudo first-order kinetic conditions using 125I-labeled galanin in at least 10-fold excess over the receptor concentration. In binding studies with neuropeptides, one has to determine whether the binding versus time curve shows a maximum followed by decline or whether saturation is achieved when incubation time increases. N-terminal fragment, which is fully conserved with respect to amino acid sequence among rat, porcine, and bovine galanin, is a high-affinity agonist at pancreatic and at hippocampal galanin receptors. The only reported cases where C-terminal galanin fragments are active alone or together with N-terminal ones concern the gastric smooth muscle in rat." @default.
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- W1044882772 date "1991-01-01" @default.
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- W1044882772 title "Assay for Galanin Receptor" @default.
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- W1044882772 doi "https://doi.org/10.1016/b978-0-12-185259-7.50020-x" @default.
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