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- W104604325 abstract "Peptide mass fingerprinting is a high throughput protein identification technique in which an unknown protein is digested with endoprotease to yield the constituent small peptides. The accurate mass of these peptides is determined by MS analysis. This gives the peak list of peptides of the unknown protein. This peak list is compared with the theoretical peptide peak list obtained from the in silico digestion of the database proteins and the best match is identified by computer software. The main advantage of this method is that it does not depend on protein sequencing for protein identification. The limitation of this method is that it requires the database to have the protein which is already characterized on another organism." @default.
- W104604325 created "2016-06-24" @default.
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- W104604325 date "2011-01-01" @default.
- W104604325 modified "2023-09-26" @default.
- W104604325 title "Protein Identification by Peptide Mass Fingerprinting (PMF)" @default.
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- W104604325 doi "https://doi.org/10.1533/9781908818058.185" @default.
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