Matches in SemOpenAlex for { <https://semopenalex.org/work/W105644275> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W105644275 abstract "Mitochondrial (m) Ca2+ uptake occurs mainly via the Ca2+ uniporter and is highly dependent on the membrane potential (Δ Ψ m). We measured Ca2+ uptake at decreasing ΔΨ m in isolated mitochondria in the presence and absence of oligomycin (OMN) to test the effect of complex V reversal. [Ca2+]m, ΔΨm, pHm, and NADHm were measured spectrophotometrically using indo-1, rhodamine 123, BCECF fluorescence and autofluorescence, respectively, in guinea pig heart isolated mitochondria. After energizing with pyruvic acid, 0, 10, 20, 30 or 100 µM dinitrophenol (DNP) was added to stepwise reduce Δ?m, after which 80 and 130 nM [Ca2+]e was added. OMN was added in some experiments to block complex V. The effect of DNP to decrease Δ?m, was exaggerated in the presence of OMN. Partial depolarization decreased the rate of matrix Ca2+ uptake, while uptake of Ca2+ resulted in further depolarization. With partial depolarization [Ca2+]m did not reach steady state but increased despite further depolarization. OMN reversed this increase and actually caused a decrease in [Ca2+]m with greater depolarization. pHm and NADHm were also lower with OMN. Our study demonstrates the importance of 1) electrical and chemical gradients for Ca2+ uptake, and 2) a likely effect of ATP hydrolysis at complex V to support ΔΨ m even during mild, graded uncoupling. Proton leaks may lead to reversal of ATP synthesis to maintain membrane potential. NIH, AHA, VA Merit grants." @default.
- W105644275 created "2016-06-24" @default.
- W105644275 creator A5035457721 @default.
- W105644275 creator A5058600813 @default.
- W105644275 creator A5060001839 @default.
- W105644275 creator A5061756444 @default.
- W105644275 creator A5075239977 @default.
- W105644275 creator A5077430453 @default.
- W105644275 date "2009-04-01" @default.
- W105644275 modified "2023-10-14" @default.
- W105644275 title "Uncoupler induced graded mitochondrial depolarization and attenuated matrix calcium uptake are enhanced by complex V inhibition indicative of blocked ATP hydrolysis" @default.
- W105644275 doi "https://doi.org/10.1096/fasebj.23.1_supplement.508.5" @default.
- W105644275 hasPublicationYear "2009" @default.
- W105644275 type Work @default.
- W105644275 sameAs 105644275 @default.
- W105644275 citedByCount "0" @default.
- W105644275 crossrefType "journal-article" @default.
- W105644275 hasAuthorship W105644275A5035457721 @default.
- W105644275 hasAuthorship W105644275A5058600813 @default.
- W105644275 hasAuthorship W105644275A5060001839 @default.
- W105644275 hasAuthorship W105644275A5061756444 @default.
- W105644275 hasAuthorship W105644275A5075239977 @default.
- W105644275 hasAuthorship W105644275A5077430453 @default.
- W105644275 hasConcept C112243037 @default.
- W105644275 hasConcept C11701378 @default.
- W105644275 hasConcept C12554922 @default.
- W105644275 hasConcept C141315368 @default.
- W105644275 hasConcept C171357686 @default.
- W105644275 hasConcept C178790620 @default.
- W105644275 hasConcept C181199279 @default.
- W105644275 hasConcept C181911157 @default.
- W105644275 hasConcept C185592680 @default.
- W105644275 hasConcept C23265538 @default.
- W105644275 hasConcept C2779134199 @default.
- W105644275 hasConcept C35341161 @default.
- W105644275 hasConcept C4141045 @default.
- W105644275 hasConcept C519063684 @default.
- W105644275 hasConcept C55493867 @default.
- W105644275 hasConcept C86803240 @default.
- W105644275 hasConcept C98539663 @default.
- W105644275 hasConceptScore W105644275C112243037 @default.
- W105644275 hasConceptScore W105644275C11701378 @default.
- W105644275 hasConceptScore W105644275C12554922 @default.
- W105644275 hasConceptScore W105644275C141315368 @default.
- W105644275 hasConceptScore W105644275C171357686 @default.
- W105644275 hasConceptScore W105644275C178790620 @default.
- W105644275 hasConceptScore W105644275C181199279 @default.
- W105644275 hasConceptScore W105644275C181911157 @default.
- W105644275 hasConceptScore W105644275C185592680 @default.
- W105644275 hasConceptScore W105644275C23265538 @default.
- W105644275 hasConceptScore W105644275C2779134199 @default.
- W105644275 hasConceptScore W105644275C35341161 @default.
- W105644275 hasConceptScore W105644275C4141045 @default.
- W105644275 hasConceptScore W105644275C519063684 @default.
- W105644275 hasConceptScore W105644275C55493867 @default.
- W105644275 hasConceptScore W105644275C86803240 @default.
- W105644275 hasConceptScore W105644275C98539663 @default.
- W105644275 hasIssue "S1" @default.
- W105644275 hasLocation W1056442751 @default.
- W105644275 hasOpenAccess W105644275 @default.
- W105644275 hasPrimaryLocation W1056442751 @default.
- W105644275 hasRelatedWork W1495324085 @default.
- W105644275 hasRelatedWork W1874604834 @default.
- W105644275 hasRelatedWork W1969389143 @default.
- W105644275 hasRelatedWork W2022856092 @default.
- W105644275 hasRelatedWork W2070663098 @default.
- W105644275 hasRelatedWork W2086101155 @default.
- W105644275 hasRelatedWork W2141174309 @default.
- W105644275 hasRelatedWork W2162575857 @default.
- W105644275 hasRelatedWork W2320220557 @default.
- W105644275 hasRelatedWork W2415549374 @default.
- W105644275 hasVolume "23" @default.
- W105644275 isParatext "false" @default.
- W105644275 isRetracted "false" @default.
- W105644275 magId "105644275" @default.
- W105644275 workType "article" @default.