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- W10565553 abstract "This chapter describes protein A plaque method. The plaque assay has allowed determinations of absolute frequencies of B cells producing Ig of a given type, class, subclass, and specificity among all Ig-secreting cells and, therefore, determinations of B cell repertoires. The method has finally been expanded to detect all Ig-secreting cells regardless of type or class of Ig. This was achieved by coupling protein A to red cells. The addition of protein A-binding, complement-binding and -activating antibodies, allows the formation of complexes between secreted Ig, specific antibody, and complement, which bind to protein A on the surface of the red cell and lyse it. With this plaque assay, it is possible to detect ceils secreting any molecules, which form complexes with their specific antibodies. Thus, this method has also been applied to the detection of cells secreting lactoferrin, myeloperoxidase, lysozyme, and albumin." @default.
- W10565553 created "2016-06-24" @default.
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- W10565553 date "1987-01-01" @default.
- W10565553 modified "2023-09-25" @default.
- W10565553 title "Protein a plaque assay" @default.
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- W10565553 doi "https://doi.org/10.1016/0076-6879(87)50079-9" @default.
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