Matches in SemOpenAlex for { <https://semopenalex.org/work/W106082014> ?p ?o ?g. }
Showing items 1 to 56 of
56
with 100 items per page.
- W106082014 abstract "N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) has been shown to cause changes in cultured endothelial cells and smooth muscle function at concentrations from 5 to 25 mM. To determine whether HEPES also affects vascular permeability, the effects of two buffers, HEPES and phosphate, were compared in isolated perfused rabbit lungs. Hemodynamic parameters and vascular protein permeability-surface area products (PS) were measured after perfusion with the buffers. Endothelial permeability was measured for an anionic and a cationic albumin to assess the charge effects of the zwitterion buffer. With HEPES, there were no changes in vascular pressure or resistance but permeability was affected. Cationic albumin permeability increased with 12 mM HEPES (8.7phosphate-->30(12) mM HEPES x ml.min-1.g dry lung-1 x 10(-2)) as did the anionic albumin PS (2.7phosphate-->3.52(12) mM HEPES). The cationic PS returned to baseline (8.1(60) mM HEPES) at 60 mM HEPES, but the anionic PS did not change from the 12 mM HEPES (4.01(60) mM HEPES). In summary, we find that HEPES is not innocuous. Although hemodynamic parameters did not change, endothelial permeability was increased when HEPES was used at normal concentrations. Therefore, HEPES should be used with caution as a physiological buffer in perfused organ systems." @default.
- W106082014 created "2016-06-24" @default.
- W106082014 creator A5026677199 @default.
- W106082014 creator A5043489015 @default.
- W106082014 creator A5057159073 @default.
- W106082014 date "1993-09-01" @default.
- W106082014 modified "2023-09-27" @default.
- W106082014 title "HEPES buffer perfusate alters rabbit lung endothelial permeability" @default.
- W106082014 doi "https://doi.org/10.1152/jappl.1993.75.3.1423" @default.
- W106082014 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8226560" @default.
- W106082014 hasPublicationYear "1993" @default.
- W106082014 type Work @default.
- W106082014 sameAs 106082014 @default.
- W106082014 citedByCount "7" @default.
- W106082014 crossrefType "journal-article" @default.
- W106082014 hasAuthorship W106082014A5026677199 @default.
- W106082014 hasAuthorship W106082014A5043489015 @default.
- W106082014 hasAuthorship W106082014A5057159073 @default.
- W106082014 hasConcept C12554922 @default.
- W106082014 hasConcept C126322002 @default.
- W106082014 hasConcept C146957229 @default.
- W106082014 hasConcept C181199279 @default.
- W106082014 hasConcept C185592680 @default.
- W106082014 hasConcept C2776816875 @default.
- W106082014 hasConcept C2780865716 @default.
- W106082014 hasConcept C55493867 @default.
- W106082014 hasConcept C71924100 @default.
- W106082014 hasConcept C86803240 @default.
- W106082014 hasConceptScore W106082014C12554922 @default.
- W106082014 hasConceptScore W106082014C126322002 @default.
- W106082014 hasConceptScore W106082014C146957229 @default.
- W106082014 hasConceptScore W106082014C181199279 @default.
- W106082014 hasConceptScore W106082014C185592680 @default.
- W106082014 hasConceptScore W106082014C2776816875 @default.
- W106082014 hasConceptScore W106082014C2780865716 @default.
- W106082014 hasConceptScore W106082014C55493867 @default.
- W106082014 hasConceptScore W106082014C71924100 @default.
- W106082014 hasConceptScore W106082014C86803240 @default.
- W106082014 hasLocation W1060820141 @default.
- W106082014 hasLocation W1060820142 @default.
- W106082014 hasOpenAccess W106082014 @default.
- W106082014 hasPrimaryLocation W1060820141 @default.
- W106082014 hasRelatedWork W1990436868 @default.
- W106082014 hasRelatedWork W2020767494 @default.
- W106082014 hasRelatedWork W2049654315 @default.
- W106082014 hasRelatedWork W2069055005 @default.
- W106082014 hasRelatedWork W2071251285 @default.
- W106082014 hasRelatedWork W2120167243 @default.
- W106082014 hasRelatedWork W2150339278 @default.
- W106082014 hasRelatedWork W2170409478 @default.
- W106082014 hasRelatedWork W2247640687 @default.
- W106082014 hasRelatedWork W2468344625 @default.
- W106082014 isParatext "false" @default.
- W106082014 isRetracted "false" @default.
- W106082014 magId "106082014" @default.
- W106082014 workType "article" @default.