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- W106318537 abstract "LLC-PK{sub 1} cells contain a well-characterized Na{sup +}-H{sup +} antiporter that is sensitive to ethylisopropylamiloride (EIPA) in the submicromolar range. Using a modification of the method of Franchi et al., the authors have selected mutants that can recover from an acid load in the presence of EIPA. One such mutant, designated PKE20, has been studied in detail. The maximal velocity (V{sub max}) for the Na{sup +}-H{sup +} antiporter, assayed as EIPA-sensitive {sup 22}Na{sup +} uptake, has increased from 44 to 106 nmol{center dot}min{sup {minus}1}{center dot}10{sup 6} cells{sup {minus}1} in PKE20. No detactable change has occurred in the K{sub m} for Na{sup +} or in the dependence of Na{sup +} uptake on intracellular pH. However, the PKE20 antiporter exhibits a greatly decreased sensitivity to amiloride and its derivatives, with drops in inhibitory potency ranging from 25-fold (amiloride) to 100-fold (EIPA). The mutation is specific for the antiporter; measurements of Na{sup +}-K{sup +} pump and Na{sup +}-dependent amino acid uptake show only small changes, which appear to result from minor antiporter-induced alterations in internal Na{sup +} concentration. PKE20 cells should prove useful in experiments to identify and isolate the antiporter protein." @default.
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- W106318537 date "1988-10-01" @default.
- W106318537 modified "2023-09-26" @default.
- W106318537 title "LLC-PK sub 1 mutant with increased Na sup + -H sup + exchange and decreased sensitivity to amiloride" @default.
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