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- W106470263 startingPage "279" @default.
- W106470263 abstract "Abstract: The taxonomy of mycotoxigenic fungi is complicated, especially within the genera Penicillium, Aspergillus, Alternaria and Fusarium, and classification is still in a state of flux. Furthermore new species are still being described. Since a polyphasic classification of filamentous fungi is generally recommended, a polyphasic identification is also recommended, even though keys based on such an approach are not yet fully developed. For practical reasons the following practice is recommended. First, fungal isolates involved in mycotoxicoses are identified to genus level using morphological criteria. Depending on the genus, isolates are then grown on a battery of indicative media and identified to species level based on a combination of morphological, physiological, nutritional and chemical data. The identification is then validated by a blast search of the DNA sequence of the β-tubulin and calmodulin genes (or other house-keeping genes with a good resolution at the species level), but avoiding ribosomal DNA genes that often have a poor resolution at the species level. Several books published in 2009 and 2010 will assist in a proper identification and established connections between species and mycotoxins will also help in avoiding misidentification. Unusual records should be retested using more taxonomic features or sequencing of more genes in order to be sure that the identification is correct." @default.
- W106470263 created "2016-06-24" @default.
- W106470263 creator A5021559678 @default.
- W106470263 date "2011-01-01" @default.
- W106470263 modified "2023-09-23" @default.
- W106470263 title "Rationale for a polyphasic approach in the identification of mycotoxigenic fungi" @default.
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- W106470263 doi "https://doi.org/10.1533/9780857090973.4.279" @default.
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