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- W107399619 abstract "The effect of three concentrations of five different cytokinins, i.e. 6-benzylamino purine, 2-isopentyl adenine,kinetin (Kin), thidiazuron and N-(2-chloro-4-pyridyl)-N-phenylurea (4-CPPU), was evaluated on the in vitro directshoot organogenesis of epicotyl explants of sour orange (Citrus aurantium L.). The basal medium used was that ofMurashige and Tucker and epicotyl explants were incubated in medium supplemented with the prementioned cytokininsfor 45 days. The addition of Kin and 4-CPPU in the medium enhanced the direct shoot organogenesis of sour orangeepicotyl segments. The concentration of each of these two cytokinins which gave the best results, was combined withindole-3-acetic acid (IAA) or a-naphthalene acetic acid (a-NAA) at concentrations ranging from 0.01 mg L�1 to 0.2mg L�1. The inclusion of IAA at 0.2 mg L�1 in the medium with 4-CPPU at 0.05 mg L�1 resulted in 100% successfuldirect shoot organogenesis, while the combination of Kin at 0.25 mg L�1 with IAA or a-NAA each at 0.01 mg L�1presented equally high organogenesis percentages (91.7%). The incubation of the produced shoots, in mediumsupplemented with either indole-3-butyric acid or a-NAA resulted in high rooting percentages (up to 90%) and therooted explants were successfully acclimatized under mist (85%). Although 4-CPPU has been used in in vitro cultureof various species, this is the first report on its use in the direct shoot organogenesis of citrus species and could be ofgreat value in citrus genetic transformation protocols using epicotyl segments, since this cytokinin resulted in theabsolute organogenesis percentage." @default.
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- W107399619 date "2011-01-01" @default.
- W107399619 modified "2023-09-26" @default.
- W107399619 title "N-(2-chloro-4-pyridyl)-N-phenylurea (4-CPPU) enhances in vitro direct shoot organogenesis of Citrus aurantium L. epicotyl segments compared to other commonly used cytokinins: short comunication" @default.
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