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- W107613089 abstract "ABSTRACT Mitochondrial (mt) DNA from Paramecium is a linear molecule, 14 μm in length (1). Replication is initiated by closure at a unique end, followed by unidirectional synthesis via a lariat intermediate, terminating in a head-to-head linear dimer (1,2). The processing of the linear dimer to two semi-conservatively constituted (3) monomer length molecules can be blocked by growth in the presence of chloramphenicol (1,2). This allows us to identify readily the initiation segment of DNA by the use of appropriate restriction endonucleases. Several species of Paramecium exist and by microinjection of isolated mitochondria, Beale and Knowles (4) have shown that species 1, 5 or 7 mitochondria can function and replicate in the nuclear environment of each other, although to different extents, but that species 4 can neither support the growth of mitochondria from the other species nor serve as a successful donor for microinjection of its mitochondria into the other species." @default.
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- W107613089 date "1979-01-01" @default.
- W107613089 modified "2023-09-27" @default.
- W107613089 title "Restriction Enzyme Analysis of Mitochondrial DNA from Closely Related Species of Paramecium11This work was supported by USPHS Grant GM 21948" @default.
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- W107613089 doi "https://doi.org/10.1016/b978-0-12-198780-0.50008-8" @default.
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