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• W108624925 abstract "Research Article1 September 1986free access Stress-induced cruciform formation in a cloned d(CATG)10 sequence. L.H. Naylor L.H. Naylor Search for more papers by this author D.M. Lilley D.M. Lilley Search for more papers by this author J.H. van de Sande J.H. van de Sande Search for more papers by this author L.H. Naylor L.H. Naylor Search for more papers by this author D.M. Lilley D.M. Lilley Search for more papers by this author J.H. van de Sande J.H. van de Sande Search for more papers by this author Author Information L.H. Naylor, D.M. Lilley and J.H. Sande The EMBO Journal (1986)5:2407-2413https://doi.org/10.1002/j.1460-2075.1986.tb04511.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The synthetic alternating purine-pyrimidine sequence, d(CATG)10.d(CATG)10, has been cloned into a 2.079-kb pBR322-derived plasmid (pLN1) and its conformation studied under torsional stress. The resultant plasmid, pLNc40, is hypersensitive to cleavage by the single strand-specific nucleases, S1 nuclease and Bal31 nuclease, and to modification by the single strand-selective reagent, osmium tetroxide. The S1-hypersensitive site of this plasmid predominates over those previously mapped in pBR322. Site-specific cleavage of pLNc40 with the resolvase T4 endonuclease VII demonstrates that this alternating purine-pyrimidine tract selectively forms a cruciform structure when stably integrated into a negatively supercoiled plasmid. Quantitative measurements of the twist change (-4.3 +/- 0.2) and free energy of formation (16.2 +/- 0.5 kcal/mol) of this cruciform have been made from two-dimensional gel electrophoresis experiments, and correspond well with the predicted values of cruciform formation for this sequence. We conclude that cruciform extrusion versus the B-Z transition is the favoured conformation of this insert under torsional stress. Previous ArticleNext Article Volume 5Issue 91 September 1986In this issue RelatedDetailsLoading ..." @default.
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• W108624925 date "1986-09-01" @default.
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• W108624925 title "Stress-induced cruciform formation in a cloned d(CATG)10 sequence." @default.
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• W108624925 doi "https://doi.org/10.1002/j.1460-2075.1986.tb04511.x" @default.
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