Matches in SemOpenAlex for { <https://semopenalex.org/work/W109103218> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W109103218 endingPage "5" @default.
- W109103218 startingPage "412" @default.
- W109103218 abstract "To study the purification, refolding and bioactivity of streptavidin-tagged human tumor necrosis factor-alpha (SA-TNF-alpha) bi-functional fusion protein.SA-TNF-alpha fusion protein was expressed in BL21(DE3) host bacteria, purified using Ni-NTA affinity chromatography and refolded by dilution and dialysis followed by identification using Western blotting. The effect of SA-TNF-alpha fusion protein against L929 cells was evaluated by MTT assay. Flow cytometry was used to analyze the surface modification of biotinylated MB49 tumor cells by SA-TNF-alpha fusion protein.Recombinant SA- TNF-alpha fusion protein was expressed in BL21(DE3) at about 30% of total bacterial protein, with a purity of about 95% after purification. The SA-TNF-alpha fusion protein existed as dimmers, tetramers and higher order structures after refolding. The fusion protein exhibited a bi-functionality by inhibiting L929 cells and SA-mediated high-affinity binding to biotinylated cell surfaces, with an anchor modification rate of above 90%.The dimmers, tetramers and higher order structures of the obtained SA-TNF-alpha fusion protein all exhibit a bi-functionality, and may serve as a potential candidate therapeutic agent for tumors." @default.
- W109103218 created "2016-06-24" @default.
- W109103218 creator A5004143556 @default.
- W109103218 creator A5040141572 @default.
- W109103218 creator A5078213280 @default.
- W109103218 creator A5088772562 @default.
- W109103218 date "2009-03-01" @default.
- W109103218 modified "2023-09-23" @default.
- W109103218 title "[Expression, purification and refolding of streptavidin-tagged human tumor necrosis factor-alpha fusion protein]." @default.
- W109103218 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19304513" @default.
- W109103218 hasPublicationYear "2009" @default.
- W109103218 type Work @default.
- W109103218 sameAs 109103218 @default.
- W109103218 citedByCount "1" @default.
- W109103218 countsByYear W1091032182012 @default.
- W109103218 crossrefType "journal-article" @default.
- W109103218 hasAuthorship W109103218A5004143556 @default.
- W109103218 hasAuthorship W109103218A5040141572 @default.
- W109103218 hasAuthorship W109103218A5078213280 @default.
- W109103218 hasAuthorship W109103218A5088772562 @default.
- W109103218 hasConcept C104317684 @default.
- W109103218 hasConcept C123894998 @default.
- W109103218 hasConcept C153911025 @default.
- W109103218 hasConcept C17991360 @default.
- W109103218 hasConcept C185592680 @default.
- W109103218 hasConcept C203014093 @default.
- W109103218 hasConcept C204628709 @default.
- W109103218 hasConcept C2776190903 @default.
- W109103218 hasConcept C2778204606 @default.
- W109103218 hasConcept C40767141 @default.
- W109103218 hasConcept C553184892 @default.
- W109103218 hasConcept C55493867 @default.
- W109103218 hasConcept C86803240 @default.
- W109103218 hasConceptScore W109103218C104317684 @default.
- W109103218 hasConceptScore W109103218C123894998 @default.
- W109103218 hasConceptScore W109103218C153911025 @default.
- W109103218 hasConceptScore W109103218C17991360 @default.
- W109103218 hasConceptScore W109103218C185592680 @default.
- W109103218 hasConceptScore W109103218C203014093 @default.
- W109103218 hasConceptScore W109103218C204628709 @default.
- W109103218 hasConceptScore W109103218C2776190903 @default.
- W109103218 hasConceptScore W109103218C2778204606 @default.
- W109103218 hasConceptScore W109103218C40767141 @default.
- W109103218 hasConceptScore W109103218C553184892 @default.
- W109103218 hasConceptScore W109103218C55493867 @default.
- W109103218 hasConceptScore W109103218C86803240 @default.
- W109103218 hasIssue "3" @default.
- W109103218 hasLocation W1091032181 @default.
- W109103218 hasOpenAccess W109103218 @default.
- W109103218 hasPrimaryLocation W1091032181 @default.
- W109103218 hasRelatedWork W1979514042 @default.
- W109103218 hasRelatedWork W2074180437 @default.
- W109103218 hasRelatedWork W2094976805 @default.
- W109103218 hasRelatedWork W2185785770 @default.
- W109103218 hasRelatedWork W2267710533 @default.
- W109103218 hasRelatedWork W2355411815 @default.
- W109103218 hasRelatedWork W2356289668 @default.
- W109103218 hasRelatedWork W2357419405 @default.
- W109103218 hasRelatedWork W2357675544 @default.
- W109103218 hasRelatedWork W2361790890 @default.
- W109103218 hasRelatedWork W2374068364 @default.
- W109103218 hasRelatedWork W2379834681 @default.
- W109103218 hasRelatedWork W2380840369 @default.
- W109103218 hasRelatedWork W2385873988 @default.
- W109103218 hasRelatedWork W2386246940 @default.
- W109103218 hasRelatedWork W2419567900 @default.
- W109103218 hasRelatedWork W2468174794 @default.
- W109103218 hasRelatedWork W3113228129 @default.
- W109103218 hasRelatedWork W3144332370 @default.
- W109103218 hasRelatedWork W3151556832 @default.
- W109103218 hasVolume "29" @default.
- W109103218 isParatext "false" @default.
- W109103218 isRetracted "false" @default.
- W109103218 magId "109103218" @default.
- W109103218 workType "article" @default.