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• W10933182 abstract "Recent works have demonstrated the experimental feasibility of real-time gene expression control based on deterministic controllers. By taking control of the level of intracellular proteins, one can probe single-cell dynamics with unprecedented flexibility. However, single-cell dynamics are stochastic in nature, and a control framework explicitly accounting for this variability is presently lacking. Here we devise a stochastic control framework, based on Model Predictive Control, which fills this gap. Based on a stochastic modelling of the gene response dynamics, our approach combines a full state-feedback receding-horizon controller with a real-time estimation method that compensates for unobserved state variables. Using previously developed models of osmostress-inducible gene expression in yeast, we show in silico that our stochastic control approach outperforms deterministic control design in the regulation of single cells. The present new contribution leads to envision the application of the proposed framework to wetlab experiments on yeast." @default.
• W10933182 created "2016-06-24" @default.
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• W10933182 date "2014-01-01" @default.
• W10933182 modified "2023-09-23" @default.
• W10933182 title "Towards Real-Time Control of Gene Expression at the Single Cell Level: A Stochastic Control Approach" @default.
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• W10933182 doi "https://doi.org/10.1007/978-3-319-12982-2_12" @default.
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