Matches in SemOpenAlex for { <https://semopenalex.org/work/W109700311> ?p ?o ?g. }
- W109700311 endingPage "174" @default.
- W109700311 startingPage "165" @default.
- W109700311 abstract "The adhesive proteins in the desmosome type of cell junction consist of two members of the cadherin superfamily, the desmogleins and desmocollins. Both desmogleins and desmocollins occur as at least three different isoforms with various patterns of expression. The molecular mechanisms controlling the differential expression of the desmosomal cadherin isoforms are not yet known. We have begun an investigation of desmoglein gene expression by cloning and analysing the promoters of the human genes coding for the type 1 and type 3 desmogleins (DSG1 and DSG3). The type 1 isoform is restricted to the suprabasal layers of the epidermis and is the autoantigen in the autoimmune blistering skin disease pemphigus foliaceous. The type 3 desmoglein isoform is also expressed in the epidermis, but in lower layers than the type 1 isoform, and is the autoantigen in pemphigus vulgaris. Phage lambda genomic clones were obtained containing 4.2 kb upstream of the translation start site of DSG1 and 517 bp upstream of the DSG3 start site. Sequencing of 660 bp upstream of DSG1 and 517 bp upstream of DSG3 revealed that there was no obvious TATA box, but a possible CAAT box was present at -238 in DSG1 and at -193 in DSG3 relative to the translation start site. Primer extension analysis and RNase protection experiments revealed four putative transcription initiation sites for DSG1 at positions -163, -151, -148 and -141, and seven closely linked sites for DSG3, the longest being at -140 relative to the translation start site. The sequences at these possible sites at -166 to -159 in DSG1 (TTCAGTCC) and at -124 to -117 in DSG3 (CTTAGACT) have some similarity to the initiator sequence (CTCANTCT) described for a TATA-less promoter often from -3 to +5, and the true transcription initiator site might therefore be the A residue in these sequences. There were two regions of similarity between the DSG1 and DSG3 promoters just upstream of the transcription initiation sites, of 20 and 13 bp, separated by 41 bp in DSG1 and 36 bp in DSG3. The significance of these regions of similarity remains to be elucidated, but the results suggest that they represent a point at which these two desmoglein genes are co-ordinately regulated. Analysis of the upstream sequences revealed GC-rich regions and consensus binding sites for transcription factors including AP-1 and AP-2. Exon boundaries were conserved compared with the classical cadherin E-cadherin, but the equivalent of the second cadherin intron was lacking. A 4.2 kb region of the human DSG1 promoter sequence was linked to the lacZ gene reporter gene in such a way that there was only one translation start site, and this construct was used to generate transgenic mice. We present the first transgenic analysis of a promoter region taken from a desmosomal cadherin gene. Our results suggest that the 4.2 kb upstream region of DSG1 does not contain all the regulatory elements necessary for correct expression of this gene but might have elements that regulate activity during hair growth." @default.
- W109700311 created "2016-06-24" @default.
- W109700311 creator A5004713618 @default.
- W109700311 creator A5005338892 @default.
- W109700311 creator A5017561809 @default.
- W109700311 creator A5020120623 @default.
- W109700311 creator A5028970290 @default.
- W109700311 creator A5039792053 @default.
- W109700311 creator A5073699632 @default.
- W109700311 creator A5084729140 @default.
- W109700311 creator A5087080060 @default.
- W109700311 date "1998-01-01" @default.
- W109700311 modified "2023-10-17" @default.
- W109700311 title "Characterization of the regulatory regions in the human desmoglein genes encoding the pemphigus foliaceous and pemphigus vulgaris antigens" @default.
- W109700311 cites W1580811574 @default.
- W109700311 cites W1594238838 @default.
- W109700311 cites W1624717390 @default.
- W109700311 cites W1860728352 @default.
- W109700311 cites W1868042911 @default.
- W109700311 cites W1963797029 @default.
- W109700311 cites W1966163497 @default.
- W109700311 cites W1969591502 @default.
- W109700311 cites W1972759188 @default.
- W109700311 cites W1973862215 @default.
- W109700311 cites W1976785613 @default.
- W109700311 cites W1977552066 @default.
- W109700311 cites W1977636211 @default.
- W109700311 cites W1978049780 @default.
- W109700311 cites W1979374048 @default.
- W109700311 cites W1985073699 @default.
- W109700311 cites W1990205754 @default.
- W109700311 cites W1996856448 @default.
- W109700311 cites W1997829811 @default.
- W109700311 cites W1999156595 @default.
- W109700311 cites W2002340643 @default.
- W109700311 cites W2008920591 @default.
- W109700311 cites W2018576877 @default.
- W109700311 cites W2020824604 @default.
- W109700311 cites W2028334176 @default.
- W109700311 cites W2035135968 @default.
- W109700311 cites W2036280725 @default.
- W109700311 cites W2036957298 @default.
- W109700311 cites W2043481572 @default.
- W109700311 cites W2051205016 @default.
- W109700311 cites W2052484848 @default.
- W109700311 cites W2058450929 @default.
- W109700311 cites W2063271223 @default.
- W109700311 cites W2068681523 @default.
- W109700311 cites W2068908390 @default.
- W109700311 cites W2069054221 @default.
- W109700311 cites W2074063192 @default.
- W109700311 cites W2077463637 @default.
- W109700311 cites W2080993852 @default.
- W109700311 cites W2082388997 @default.
- W109700311 cites W2092743129 @default.
- W109700311 cites W2108631052 @default.
- W109700311 cites W2118094114 @default.
- W109700311 cites W2118299921 @default.
- W109700311 cites W2119033016 @default.
- W109700311 cites W2125052153 @default.
- W109700311 cites W2131186777 @default.
- W109700311 cites W2133462524 @default.
- W109700311 cites W2142808792 @default.
- W109700311 cites W2150104849 @default.
- W109700311 cites W2289947747 @default.
- W109700311 doi "https://doi.org/10.1042/bj3290165" @default.
- W109700311 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1219028" @default.
- W109700311 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9405290" @default.
- W109700311 hasPublicationYear "1998" @default.
- W109700311 type Work @default.
- W109700311 sameAs 109700311 @default.
- W109700311 citedByCount "32" @default.
- W109700311 countsByYear W1097003112014 @default.
- W109700311 countsByYear W1097003112015 @default.
- W109700311 countsByYear W1097003112016 @default.
- W109700311 countsByYear W1097003112021 @default.
- W109700311 countsByYear W1097003112022 @default.
- W109700311 crossrefType "journal-article" @default.
- W109700311 hasAuthorship W109700311A5004713618 @default.
- W109700311 hasAuthorship W109700311A5005338892 @default.
- W109700311 hasAuthorship W109700311A5017561809 @default.
- W109700311 hasAuthorship W109700311A5020120623 @default.
- W109700311 hasAuthorship W109700311A5028970290 @default.
- W109700311 hasAuthorship W109700311A5039792053 @default.
- W109700311 hasAuthorship W109700311A5073699632 @default.
- W109700311 hasAuthorship W109700311A5084729140 @default.
- W109700311 hasAuthorship W109700311A5087080060 @default.
- W109700311 hasBestOaLocation W1097003112 @default.
- W109700311 hasConcept C104317684 @default.
- W109700311 hasConcept C1491633281 @default.
- W109700311 hasConcept C153911025 @default.
- W109700311 hasConcept C159654299 @default.
- W109700311 hasConcept C163764329 @default.
- W109700311 hasConcept C203014093 @default.
- W109700311 hasConcept C2778757060 @default.
- W109700311 hasConcept C2778862538 @default.
- W109700311 hasConcept C2781313415 @default.
- W109700311 hasConcept C2909223747 @default.