FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W110103163> ?p ?o ?g. }

• W110103163 endingPage "119" @default.
• W110103163 startingPage "112" @default.
• W110103163 abstract "The interaction of the novel cationic porphyrin – imidazophenazine conjugate (TMP3-Pzn), as well as its zinc (II) and manganese (III) metallocomplexes  with intramolecular G-quadruplex formed by 22-mer oligodeoxynucleotide d[AGGG(TTAGGG)3] of human telomeric repeat (22G4) was studied.  Techniques of absorption and polarized fluorescent spectroscopy as well as absorption melting at 295 nm were used. The binding affinity of the conjugates to the quadruplex was studied using titration experiments registering the dependence of the fluorescence intensity and polarization degree for porphyrin moieties  on quadruplex/ligand molar ratio (P/D). All investigations were carried out in 2 mM phosphate buffer (pH6.9) containing 0.5 mM EDTA and 0.1 M NaCl. Spectroscopic characteristics of the TMP3-Pzn and ZnTMP3-Pzn conjugates evidence the formation of internal heterodimers with stacking between porphyrin and phenazine chromophores, although in the case of MnTMP3-Pzn it has not occurred. It was established that all the conjugates reveal high binding affinities to the intra-molecular G-quadruplex, probably, via external groove binding of porphyrin moiety. The complex formation is accompanied by the increase in the fluorescence intensity and polarization degree of porphyrin fragments. It should be noted that negligibly small fluorescence of manganese porphyrin moiety flames up by near 20 times under the binding, being the novel observation for manganese complex. It was found that ZnTMP3-Pzn and MnTMP3-Pzn stabilize the G-quadruplex structure increasing the transition midpoint temperature by 3 °C, whereas non-metallated TMP3-Pzn conjugate decreases it by approximately 2 °C. Despite of the opposite changes in G-quadruplex melting temperature, a simultaneous increase in the transition enthalpy and entropy was determined for all conjugates, which gives a some stabilizing increment in the Gibbs standard free energy. In the case of metal complexes, 10-fold increase in the 22G4 folding equilibrium constant at 37 °C was determined" @default.
• W110103163 created "2016-06-24" @default.
• W110103163 creator A5011315780 @default.
• W110103163 creator A5021609853 @default.
• W110103163 creator A5038238211 @default.
• W110103163 creator A5081715022 @default.
• W110103163 creator A5084718927 @default.
• W110103163 date "2011-08-30" @default.
• W110103163 modified "2023-10-01" @default.
• W110103163 title "Spectroscopic Studies on Binding of Porphyrin-Phenazine Conjugate to Intramolecular G-Quadruplex Formed by 22-mer Oligonucleotide" @default.
• W110103163 cites W1971471663 @default.
• W110103163 cites W1998439254 @default.
• W110103163 cites W1998663807 @default.
• W110103163 cites W2004976345 @default.
• W110103163 cites W2067184067 @default.
• W110103163 cites W2076079790 @default.
• W110103163 cites W2079768304 @default.
• W110103163 cites W2139789471 @default.
• W110103163 cites W2908999615 @default.
• W110103163 doi "https://doi.org/10.15866/irebic.v2i4.1522" @default.
• W110103163 hasPublicationYear "2011" @default.
• W110103163 type Work @default.
• W110103163 sameAs 110103163 @default.
• W110103163 citedByCount "1" @default.
• W110103163 crossrefType "journal-article" @default.
• W110103163 hasAuthorship W110103163A5011315780 @default.
• W110103163 hasAuthorship W110103163A5021609853 @default.
• W110103163 hasAuthorship W110103163A5038238211 @default.
• W110103163 hasAuthorship W110103163A5081715022 @default.
• W110103163 hasAuthorship W110103163A5084718927 @default.
• W110103163 hasConcept C121332964 @default.
• W110103163 hasConcept C133571119 @default.
• W110103163 hasConcept C134306372 @default.
• W110103163 hasConcept C178790620 @default.
• W110103163 hasConcept C185592680 @default.
• W110103163 hasConcept C192468462 @default.
• W110103163 hasConcept C197336794 @default.
• W110103163 hasConcept C2776568683 @default.
• W110103163 hasConcept C2778370995 @default.
• W110103163 hasConcept C2779480358 @default.
• W110103163 hasConcept C33347731 @default.
• W110103163 hasConcept C33923547 @default.
• W110103163 hasConcept C62520636 @default.
• W110103163 hasConcept C71240020 @default.
• W110103163 hasConcept C75079739 @default.
• W110103163 hasConcept C75473681 @default.
• W110103163 hasConcept C8010536 @default.
• W110103163 hasConcept C91881484 @default.
• W110103163 hasConceptScore W110103163C121332964 @default.
• W110103163 hasConceptScore W110103163C133571119 @default.
• W110103163 hasConceptScore W110103163C134306372 @default.
• W110103163 hasConceptScore W110103163C178790620 @default.
• W110103163 hasConceptScore W110103163C185592680 @default.
• W110103163 hasConceptScore W110103163C192468462 @default.
• W110103163 hasConceptScore W110103163C197336794 @default.
• W110103163 hasConceptScore W110103163C2776568683 @default.
• W110103163 hasConceptScore W110103163C2778370995 @default.
• W110103163 hasConceptScore W110103163C2779480358 @default.
• W110103163 hasConceptScore W110103163C33347731 @default.
• W110103163 hasConceptScore W110103163C33923547 @default.
• W110103163 hasConceptScore W110103163C62520636 @default.
• W110103163 hasConceptScore W110103163C71240020 @default.
• W110103163 hasConceptScore W110103163C75079739 @default.
• W110103163 hasConceptScore W110103163C75473681 @default.
• W110103163 hasConceptScore W110103163C8010536 @default.
• W110103163 hasConceptScore W110103163C91881484 @default.
• W110103163 hasIssue "4" @default.
• W110103163 hasLocation W1101031631 @default.
• W110103163 hasOpenAccess W110103163 @default.
• W110103163 hasPrimaryLocation W1101031631 @default.
• W110103163 hasRelatedWork W1965309393 @default.
• W110103163 hasRelatedWork W2004702561 @default.
• W110103163 hasRelatedWork W2017074745 @default.
• W110103163 hasRelatedWork W2018236752 @default.
• W110103163 hasRelatedWork W2034605243 @default.
• W110103163 hasRelatedWork W2061081099 @default.
• W110103163 hasRelatedWork W2065302768 @default.
• W110103163 hasRelatedWork W2095671674 @default.
• W110103163 hasRelatedWork W2116796181 @default.
• W110103163 hasRelatedWork W2320852218 @default.
• W110103163 hasRelatedWork W2333389715 @default.
• W110103163 hasRelatedWork W2510693980 @default.
• W110103163 hasRelatedWork W2557029390 @default.
• W110103163 hasRelatedWork W2622487782 @default.
• W110103163 hasRelatedWork W2898779360 @default.
• W110103163 hasRelatedWork W2942820246 @default.
• W110103163 hasRelatedWork W2991066949 @default.
• W110103163 hasRelatedWork W3019324488 @default.
• W110103163 hasRelatedWork W3141062504 @default.
• W110103163 hasRelatedWork W2903470137 @default.
• W110103163 hasVolume "2" @default.
• W110103163 isParatext "false" @default.
• W110103163 isRetracted "false" @default.
• W110103163 magId "110103163" @default.
• W110103163 workType "article" @default.