FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W110338051> ?p ?o ?g. }

• W110338051 endingPage "307" @default.
• W110338051 startingPage "295" @default.
• W110338051 abstract "Fluorescent tagging of proteins has become a critical step in optical analysis of protein function in vitro and in living cells. Here we describe a two-tag system for expression and isolation of a protein of interest from Escherichia coli and subsequent site-specific fluorescent labeling with Sfp phosphopantetheinyl transferase (Sfp synthase). In the example presented, adenoviral protein E3-14.7 K (E14.7) was expressed as a tripartite fusion protein with a fluorophore-targeting peptide tag and a chitin-binding domain. This system allows for rapid isolation of the recombinant fusion protein from crude bacterial cell lysate via a single chitin column. Sfp synthase-mediated labeling with fluorophore conjugated to coenzyme A-SH (CoA-SH) resulted in covalent attachment of a fluorescent dye to a specific residue of the peptide tag via a phosphopantetheinyl linker. The fluorescently labeled E14.7 fusion protein was analyzed with a fluorescence imager and subsequently transfected into mammalian cells for imaging with a fluorescence microscope." @default.
• W110338051 created "2016-06-24" @default.
• W110338051 creator A5029979178 @default.
• W110338051 creator A5030670609 @default.
• W110338051 creator A5052319792 @default.
• W110338051 creator A5053150479 @default.
• W110338051 creator A5065093852 @default.
• W110338051 creator A5082971249 @default.
• W110338051 creator A5084249243 @default.
• W110338051 creator A5087079717 @default.
• W110338051 date "2010-11-12" @default.
• W110338051 modified "2023-10-01" @default.
• W110338051 title "Fluorescent Site-Specific Labeling of Escherichia coli Expressed Proteins with Sfp Phosphopantetheinyl Transferase" @default.
• W110338051 cites W1987988513 @default.
• W110338051 cites W1994477951 @default.
• W110338051 cites W2016036293 @default.
• W110338051 cites W2041521990 @default.
• W110338051 cites W2057141116 @default.
• W110338051 cites W2071938141 @default.
• W110338051 cites W2074261421 @default.
• W110338051 cites W2105842004 @default.
• W110338051 cites W2105888055 @default.
• W110338051 cites W2106254834 @default.
• W110338051 cites W2110367525 @default.
• W110338051 cites W2127068865 @default.
• W110338051 cites W2136714897 @default.
• W110338051 cites W2150881786 @default.
• W110338051 cites W2328394317 @default.
• W110338051 cites W4293247451 @default.
• W110338051 doi "https://doi.org/10.1007/978-1-61737-967-3_18" @default.
• W110338051 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21125394" @default.
• W110338051 hasPublicationYear "2010" @default.
• W110338051 type Work @default.
• W110338051 sameAs 110338051 @default.
• W110338051 citedByCount "5" @default.
• W110338051 countsByYear W1103380512013 @default.
• W110338051 countsByYear W1103380512020 @default.
• W110338051 countsByYear W1103380512023 @default.
• W110338051 crossrefType "book-chapter" @default.
• W110338051 hasAuthorship W110338051A5029979178 @default.
• W110338051 hasAuthorship W110338051A5030670609 @default.
• W110338051 hasAuthorship W110338051A5052319792 @default.
• W110338051 hasAuthorship W110338051A5053150479 @default.
• W110338051 hasAuthorship W110338051A5065093852 @default.
• W110338051 hasAuthorship W110338051A5082971249 @default.
• W110338051 hasAuthorship W110338051A5084249243 @default.
• W110338051 hasAuthorship W110338051A5087079717 @default.
• W110338051 hasConcept C104317684 @default.
• W110338051 hasConcept C111919701 @default.
• W110338051 hasConcept C121332964 @default.
• W110338051 hasConcept C123894998 @default.
• W110338051 hasConcept C142613039 @default.
• W110338051 hasConcept C153911025 @default.
• W110338051 hasConcept C169274487 @default.
• W110338051 hasConcept C185592680 @default.
• W110338051 hasConcept C2778617687 @default.
• W110338051 hasConcept C2779281246 @default.
• W110338051 hasConcept C2780557392 @default.
• W110338051 hasConcept C40767141 @default.
• W110338051 hasConcept C41008148 @default.
• W110338051 hasConcept C547475151 @default.
• W110338051 hasConcept C55493867 @default.
• W110338051 hasConcept C62520636 @default.
• W110338051 hasConcept C86803240 @default.
• W110338051 hasConcept C91881484 @default.
• W110338051 hasConceptScore W110338051C104317684 @default.
• W110338051 hasConceptScore W110338051C111919701 @default.
• W110338051 hasConceptScore W110338051C121332964 @default.
• W110338051 hasConceptScore W110338051C123894998 @default.
• W110338051 hasConceptScore W110338051C142613039 @default.
• W110338051 hasConceptScore W110338051C153911025 @default.
• W110338051 hasConceptScore W110338051C169274487 @default.
• W110338051 hasConceptScore W110338051C185592680 @default.
• W110338051 hasConceptScore W110338051C2778617687 @default.
• W110338051 hasConceptScore W110338051C2779281246 @default.
• W110338051 hasConceptScore W110338051C2780557392 @default.
• W110338051 hasConceptScore W110338051C40767141 @default.
• W110338051 hasConceptScore W110338051C41008148 @default.
• W110338051 hasConceptScore W110338051C547475151 @default.
• W110338051 hasConceptScore W110338051C55493867 @default.
• W110338051 hasConceptScore W110338051C62520636 @default.
• W110338051 hasConceptScore W110338051C86803240 @default.
• W110338051 hasConceptScore W110338051C91881484 @default.
• W110338051 hasLocation W1103380511 @default.
• W110338051 hasLocation W1103380512 @default.
• W110338051 hasOpenAccess W110338051 @default.
• W110338051 hasPrimaryLocation W1103380511 @default.
• W110338051 hasRelatedWork W110338051 @default.
• W110338051 hasRelatedWork W1985219525 @default.
• W110338051 hasRelatedWork W1990016848 @default.
• W110338051 hasRelatedWork W2235504456 @default.
• W110338051 hasRelatedWork W2293347756 @default.
• W110338051 hasRelatedWork W2379008071 @default.
• W110338051 hasRelatedWork W2379282932 @default.
• W110338051 hasRelatedWork W2380100330 @default.
• W110338051 hasRelatedWork W2381981384 @default.
• W110338051 hasRelatedWork W2391890413 @default.
• W110338051 isParatext "false" @default.

• next