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- W110624105 abstract "Neuropeptides, which control emotion, behavior and body homeostasis, are released at nerve terminals through the exocytosis of dense-core vesicles (DCVs). Because neuropeptides are synthesized and packaged exclusively at the cell body, accurate and timely delivery of DCV to the distant nerve terminal, as well as tight regulation of neuropeptide release should be ensured to prevent the deprivation of releasable DCVs during activity. Traditionally, it was believed that DCVs are delivered to the nerve terminal exclusively through anterograde fast axonal transport. However, it is now known that DCVs continuously transit bidirectionally at nerve terminals that are highly branched with release sites located along strings of varicosities called en passant boutons. Therefore, it is not clear how DCV logistics operates to support neurosecretion. In addition, little is known about the release properties of neuropeptides in fully differentiated neurons.To address these issues, GFP-tagged neuropeptides were imaged in Drosophila melanogaster neuromuscular junction (NMJ) to study the routing and exocytosis behavior of DCVs in vivo. By using a newly developed optical technique, simultaneous photobleaching and imaging (SPAIM), to track single DCVs, it was found that DCVs circulate between the most distal bouton and the proximal axon. Neuropeptides are constantly delivered to en passant boutons via sporadic bidirectional capture of DCVs. These newly arrived DCVs can become releasable within minutes. Surprisingly, neuropeptides are discharged only partially and slowly from DCVs, suggesting that “kiss-and-run” mode of exocytosis predominates over full fusion. Interestingly, these partially emptied DCVs can undergo multiple rounds of exocytosis, allowing some local recycling of DCVs and minimizing the need for the supply of new DCVs. Finally, it was found that DCVs are reloaded into en passant boutons after depolarization evoked-neuropeptide release by activity-dependent capture that is regulated through the ryanodine receptor-CamKII signaling pathway. Altogether, this thesis describes some elegant mechanisms utilized by the neuron that efficiently maintain neuropeptide stores at the distant and morphologically complex nerve terminal." @default.
- W110624105 created "2016-06-24" @default.
- W110624105 creator A5058006251 @default.
- W110624105 date "2012-04-18" @default.
- W110624105 modified "2023-09-23" @default.
- W110624105 title "Delivery and exocytosis of neuropeptide vesicles at the nerve terminal" @default.
- W110624105 cites W100802 @default.
- W110624105 cites W1501894556 @default.
- W110624105 cites W1502026371 @default.
- W110624105 cites W1521104469 @default.
- W110624105 cites W1534597181 @default.
- W110624105 cites W1564650596 @default.
- W110624105 cites W1605314587 @default.
- W110624105 cites W169301608 @default.
- W110624105 cites W1765279797 @default.
- W110624105 cites W1856652139 @default.
- W110624105 cites W1926996067 @default.
- W110624105 cites W1968683040 @default.
- W110624105 cites W1970249565 @default.
- W110624105 cites W1970512089 @default.
- W110624105 cites W1970729216 @default.
- W110624105 cites W1975948750 @default.
- W110624105 cites W1977353559 @default.
- W110624105 cites W1979141206 @default.
- W110624105 cites W1979240985 @default.
- W110624105 cites W1981471126 @default.
- W110624105 cites W1983542762 @default.
- W110624105 cites W1983584483 @default.
- W110624105 cites W1984979116 @default.
- W110624105 cites W1987386100 @default.
- W110624105 cites W1991558060 @default.
- W110624105 cites W1992556461 @default.
- W110624105 cites W1993386867 @default.
- W110624105 cites W1995408976 @default.
- W110624105 cites W1996368170 @default.
- W110624105 cites W1996657515 @default.
- W110624105 cites W1997564419 @default.
- W110624105 cites W1998105194 @default.
- W110624105 cites W1999472605 @default.
- W110624105 cites W2000049837 @default.
- W110624105 cites W2000531537 @default.
- W110624105 cites W2001597155 @default.
- W110624105 cites W2003096362 @default.
- W110624105 cites W2004285833 @default.
- W110624105 cites W2005128877 @default.
- W110624105 cites W2007114792 @default.
- W110624105 cites W2008501176 @default.
- W110624105 cites W2008562288 @default.
- W110624105 cites W2008784560 @default.
- W110624105 cites W2009441114 @default.
- W110624105 cites W2011094752 @default.
- W110624105 cites W2011704559 @default.
- W110624105 cites W2012754839 @default.
- W110624105 cites W2014054947 @default.
- W110624105 cites W2014095197 @default.
- W110624105 cites W2015832139 @default.
- W110624105 cites W2016671569 @default.
- W110624105 cites W2016981128 @default.
- W110624105 cites W2021258666 @default.
- W110624105 cites W2021836727 @default.
- W110624105 cites W2022343725 @default.
- W110624105 cites W2023317124 @default.
- W110624105 cites W2026175547 @default.
- W110624105 cites W2027952771 @default.
- W110624105 cites W2029120000 @default.
- W110624105 cites W2029212281 @default.
- W110624105 cites W2029633475 @default.
- W110624105 cites W2029683101 @default.
- W110624105 cites W2031058780 @default.
- W110624105 cites W2032738449 @default.
- W110624105 cites W2033067408 @default.
- W110624105 cites W2033888271 @default.
- W110624105 cites W2035441746 @default.
- W110624105 cites W2035718469 @default.
- W110624105 cites W2037956598 @default.
- W110624105 cites W2038415502 @default.
- W110624105 cites W2040027787 @default.
- W110624105 cites W2040910366 @default.
- W110624105 cites W2041102021 @default.
- W110624105 cites W2041619316 @default.
- W110624105 cites W2042666230 @default.
- W110624105 cites W2044024554 @default.
- W110624105 cites W2044836636 @default.
- W110624105 cites W2047250323 @default.
- W110624105 cites W2047744098 @default.
- W110624105 cites W2047746821 @default.
- W110624105 cites W2047903878 @default.
- W110624105 cites W2049046584 @default.
- W110624105 cites W2049225918 @default.
- W110624105 cites W2051478210 @default.
- W110624105 cites W2052794108 @default.
- W110624105 cites W2053050328 @default.
- W110624105 cites W2054181228 @default.
- W110624105 cites W2054285168 @default.
- W110624105 cites W2055022577 @default.
- W110624105 cites W2055572268 @default.
- W110624105 cites W2057150795 @default.
- W110624105 cites W2058289105 @default.
- W110624105 cites W2059999294 @default.
- W110624105 cites W2063151142 @default.