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- W112272212 abstract "Publisher SummaryThis chapter discusses the techniques of chromosome analysis following short- and long-term cultures in animals, including man. It presents the principle of the use of colchicine or other mitostatic chemicals for causing metaphase arrest in both meiotic and mitotic preparations dealing with pretreatment. The dispersal of chromosomes by hypotonic shock led to the evolution of most of the methods on mammalian chromosomes, but its possible drawback is the loss of certain information, like the probable association of chromosomes with satellites between them, the somatic pairing of homologues and their spatial arrangement in the equator. With relation to the initiation of leucocyte division in the peripheral blood culture, the discovery of the plant product, phytohaemagglutin in (PHA), should no doubt be considered as a landmark in the evolution of technology. Moreover, the application of organic compounds like kinetin, gibberellin, ascorbic acid and other hormones—some of which are known to initiate division in nuclei in other biological objects—on human and mammalian tissue, may give valuable results in this direction. With further refinements in technology as envisaged above, the judicious handling of the human chromosome, if not the genes, from the pre-natal to the adult phases, may perhaps successfully aim at the betterment of the human race." @default.
- W112272212 created "2016-06-24" @default.
- W112272212 creator A5016338755 @default.
- W112272212 creator A5079136540 @default.
- W112272212 date "1980-01-01" @default.
- W112272212 modified "2023-09-27" @default.
- W112272212 title "Chromosome analysis following short- and long-term cultures in animals, including man" @default.
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