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- W112306052 abstract "LTB4 and its ω-oxidation products may be rapidly, sensitively, and specifically quantitated by the methods of solid-phase extraction and reversed-phase high-performance liquid chromatography (HPLC), which are described in this chapter. Although other techniques, such as radioimmunoassay or gas chromatography-mass spectrometry, may be utilized for quantitative analysis of the lipoxygenase products of arachidonic acid, only the technique of reversed-phase HPLC can quantitate as many as 10 metabolites in a single analysis, without prior derivatization. In this chapter, we also reviewed the chromatographic theory which we utilized in order to optimize reversed-phase HPLC analysis of LTB4 and its ω-oxidation products. With this information and a gradient HPLC system, it is possible for any investigator to develop a powerful assay for the potent inflammatory mediator, LTB4, or for any other lipoxygenase product of arachidonic acid." @default.
- W112306052 created "2016-06-24" @default.
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- W112306052 date "1987-01-01" @default.
- W112306052 modified "2023-09-25" @default.
- W112306052 title "Leukotriene B4 catabolism: Quantitation of leukotriene B4 and its ω-oxidation products by reversed-phase high-performance liquid chromatography" @default.
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- W112306052 doi "https://doi.org/10.1016/0076-6879(87)41083-5" @default.
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