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• W1124315074 abstract "This chapter presents flow-cytometric analysis of chromatin. Flow-cytometry is the rapid automated measurement of cell fluorescence. Pulse-height analysis of the induced fluorescence of individual cells in a stream permits determination of distributions of amounts of fluorescent-stained materials. The basic elements of the instrument are (1) the flow cell that contains and shapes the stream, (2) an intense light source, usually a continuous-beam argon laser, (3) a detector unit consisting of one or more photomultipliers with their power supply, and (4) a pulse-height analyzer consisting of signal amplifiers, analog digital converters, a memory, a data retrieval system, and a variety of accessories the need for which varies with the complexity of the instrument. The chapter discusses the practical considerations in analyzing chromatin by flow cytometry (FCM). The method consists of preparation of the cells, measurement, and analysis of the data. Each of these steps requires attention to problems that are unique to the cell or tissue being studied and to the method of staining used. The chapter discusses techniques in tissue dispersal, fixation and staining of chromatin in cells and nuclei, and some stains used for the flow-microfluorometric analysis of chromatin." @default.
• W1124315074 created "2016-06-24" @default.
• W1124315074 creator A5002297167 @default.
• W1124315074 creator A5071786985 @default.
• W1124315074 creator A5081168162 @default.
• W1124315074 date "1978-01-01" @default.
• W1124315074 modified "2023-10-18" @default.
• W1124315074 title "Chapter 14 Flow-Cytometric Analysis of Chromatin" @default.
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