Matches in SemOpenAlex for { <https://semopenalex.org/work/W11244721> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W11244721 endingPage "120" @default.
- W11244721 startingPage "109" @default.
- W11244721 abstract "Objective To develop a rapid and definite diagnostic test of bacterial enteritis caused by pathogenic enterobacteria, the most frequent etiologic agent of infectious enteritis in the world.Methods A set of conventional PCR assays were applied to detect and identify salmonella, shigella,and E. coli O157:H7 directly from pure culture and fecal samples. The general primers of pathogenic enterobacteria were located on the uidA gene, which were found not only in E. coli nuclear acid, but also in Shigella and salmonella genes. Shigella primer was from ipaH gene whose coded invasive plasmid relative antigen existed both in plasmid and in genome. The primers of salmonella were designed from the 16SrRNA sequence. The primer of E. coli O157:H7 was taken from eaeA gene.Five random primers were selected for RAPD. The detection system included common PCR,semi-nested PCR and RAPD. Results This method was more sensitive, specific and efficient and its processing was rapid and simple. For example, the method could be used to specifically detect and identify salmonella, shigella, and E. coli O157:H7, and its sensitivity ranged from 3 to 50 CFU, and its detection time was 4 hours. Conclusion This PCR method, therefore, can serve as a rourine and practical protocol for detecting and identifying pathogenic microorganisms from clinical samples." @default.
- W11244721 created "2016-06-24" @default.
- W11244721 creator A5039225931 @default.
- W11244721 creator A5046241226 @default.
- W11244721 creator A5047395455 @default.
- W11244721 creator A5062075430 @default.
- W11244721 creator A5075345789 @default.
- W11244721 creator A5077006621 @default.
- W11244721 date "2004-01-01" @default.
- W11244721 modified "2023-09-27" @default.
- W11244721 title "A Study on Detecting and Identifying Enteric Pathogens With PCR" @default.
- W11244721 hasPublicationYear "2004" @default.
- W11244721 type Work @default.
- W11244721 sameAs 11244721 @default.
- W11244721 citedByCount "0" @default.
- W11244721 crossrefType "journal-article" @default.
- W11244721 hasAuthorship W11244721A5039225931 @default.
- W11244721 hasAuthorship W11244721A5046241226 @default.
- W11244721 hasAuthorship W11244721A5047395455 @default.
- W11244721 hasAuthorship W11244721A5062075430 @default.
- W11244721 hasAuthorship W11244721A5075345789 @default.
- W11244721 hasAuthorship W11244721A5077006621 @default.
- W11244721 hasConcept C104317684 @default.
- W11244721 hasConcept C159047783 @default.
- W11244721 hasConcept C178790620 @default.
- W11244721 hasConcept C185592680 @default.
- W11244721 hasConcept C22744801 @default.
- W11244721 hasConcept C2776986154 @default.
- W11244721 hasConcept C2777563447 @default.
- W11244721 hasConcept C2780031333 @default.
- W11244721 hasConcept C2781065037 @default.
- W11244721 hasConcept C2781217973 @default.
- W11244721 hasConcept C2908647359 @default.
- W11244721 hasConcept C49105822 @default.
- W11244721 hasConcept C523546767 @default.
- W11244721 hasConcept C54355233 @default.
- W11244721 hasConcept C547475151 @default.
- W11244721 hasConcept C71924100 @default.
- W11244721 hasConcept C81977670 @default.
- W11244721 hasConcept C84623179 @default.
- W11244721 hasConcept C86803240 @default.
- W11244721 hasConcept C89423630 @default.
- W11244721 hasConcept C99454951 @default.
- W11244721 hasConceptScore W11244721C104317684 @default.
- W11244721 hasConceptScore W11244721C159047783 @default.
- W11244721 hasConceptScore W11244721C178790620 @default.
- W11244721 hasConceptScore W11244721C185592680 @default.
- W11244721 hasConceptScore W11244721C22744801 @default.
- W11244721 hasConceptScore W11244721C2776986154 @default.
- W11244721 hasConceptScore W11244721C2777563447 @default.
- W11244721 hasConceptScore W11244721C2780031333 @default.
- W11244721 hasConceptScore W11244721C2781065037 @default.
- W11244721 hasConceptScore W11244721C2781217973 @default.
- W11244721 hasConceptScore W11244721C2908647359 @default.
- W11244721 hasConceptScore W11244721C49105822 @default.
- W11244721 hasConceptScore W11244721C523546767 @default.
- W11244721 hasConceptScore W11244721C54355233 @default.
- W11244721 hasConceptScore W11244721C547475151 @default.
- W11244721 hasConceptScore W11244721C71924100 @default.
- W11244721 hasConceptScore W11244721C81977670 @default.
- W11244721 hasConceptScore W11244721C84623179 @default.
- W11244721 hasConceptScore W11244721C86803240 @default.
- W11244721 hasConceptScore W11244721C89423630 @default.
- W11244721 hasConceptScore W11244721C99454951 @default.
- W11244721 hasIssue "1" @default.
- W11244721 hasLocation W112447211 @default.
- W11244721 hasOpenAccess W11244721 @default.
- W11244721 hasPrimaryLocation W112447211 @default.
- W11244721 hasRelatedWork W126204657 @default.
- W11244721 hasRelatedWork W1999974762 @default.
- W11244721 hasRelatedWork W2037148198 @default.
- W11244721 hasRelatedWork W2071233016 @default.
- W11244721 hasRelatedWork W2098193356 @default.
- W11244721 hasRelatedWork W2117634096 @default.
- W11244721 hasRelatedWork W2182085958 @default.
- W11244721 hasRelatedWork W2188005644 @default.
- W11244721 hasRelatedWork W2301099699 @default.
- W11244721 hasRelatedWork W2349312179 @default.
- W11244721 hasRelatedWork W2363796151 @default.
- W11244721 hasRelatedWork W2365776297 @default.
- W11244721 hasRelatedWork W2371435784 @default.
- W11244721 hasRelatedWork W2379239590 @default.
- W11244721 hasRelatedWork W2382597736 @default.
- W11244721 hasRelatedWork W2389599546 @default.
- W11244721 hasRelatedWork W2396507205 @default.
- W11244721 hasRelatedWork W3148440530 @default.
- W11244721 hasRelatedWork W54962369 @default.
- W11244721 hasRelatedWork W122467609 @default.
- W11244721 hasVolume "17" @default.
- W11244721 isParatext "false" @default.
- W11244721 isRetracted "false" @default.
- W11244721 magId "11244721" @default.
- W11244721 workType "article" @default.