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- W112697547 abstract "The fission yeast Schizosaccharomyces pombe has many biological characteristics that make it an ideal model organism for the study of meiosis. A nearly synchronous meiosis is one of the most important. Under certain environmental and genetic conditions, large cultures of S. pombe can be induced to undergo meiosis in a timely and predictable manner that allows for changes in the DNA to be observed and analyzed by gel electrophoresis. Initiation of meiotic recombination via programmed DNA double-strand breaks, the formation of joint molecule recombination intermediates, and the resolution of these intermediates into crossover DNA products can all be seen with consistent timing during the progression of a synchronous meiotic induction. The timing of recombination events, the genetic requirements for the formation and disappearance of recombination intermediates, and the analysis of the DNA structures of those intermediates allow a comparison of meiotic recombination in fission yeast with that in the only other species similarly studied, the budding yeast Saccharomyces cerevisiae." @default.
- W112697547 created "2016-06-24" @default.
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- W112697547 date "2009-01-01" @default.
- W112697547 modified "2023-10-03" @default.
- W112697547 title "Using Schizosaccharomyces pombe Meiosis to Analyze DNA Recombination Intermediates" @default.
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- W112697547 doi "https://doi.org/10.1007/978-1-59745-527-5_15" @default.
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