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- W1129382998 abstract "Cortisol metabolites are present in the urine primarily in the conjugated form with β-glucuronic acid. In the guinea pig and some other species the metabolites are excreted unconjugated. Ethyl acetate is the solvent of choice to extract free compounds. Methylene chloride, chloroform, or ethyl ether are less suitable, because they do not extract the more polar compounds such as 6-hydroxycortisol. Cortisol metabolites can be isolated from the aqueous incubation media by adsorption onto Amberlite XAD-2, as described in this chapter. This serves to separate the steroid metabolites from small molecules (glucose, salts, urea, buffers, etc.) in the media. Recovery is usually greater than 95%. Alternatively, the media could be extracted with ethyl acetate or chloroform to obtain unconjugated compounds. Compounds present in tissues or cell preparations can be extracted with acetone or ethyl acetate. If necessary, hydrolysis can be carried out as described for urine. Although separations can be achieved by thin-layer chromatography, the separation of individual metabolites is best carried out by chromatography. Although paper chromatography is preferred by most experimenters because of its speed and relative ease, column chromatographic systems that will also separate these metabolites are described and the use of resin columns to separate these metabolites are reported in the chapter. Although fairly good separations have been achieved, the use of columns is more tedious." @default.
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- W1129382998 date "1975-01-01" @default.
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- W1129382998 title "[44] Isolation of cortisol metabolites" @default.
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- W1129382998 doi "https://doi.org/10.1016/s0076-6879(75)36047-3" @default.
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