FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W113377609> ?p ?o ?g. }

• W113377609 abstract "The development of an organism from the fertilized zygote to amulticellular organism is a unidirectional process. It occurs in a spatially andtemporally tightly controlled fashion. To understand how the genetic informationis interpreted and how the cellular identity is inherited, are major challengestowards the understanding of developmental processes. Epigenetic marks likehistone modifications, changes of the protein composition binding to DNA or theremodeling of nucleosomes have been shown to be important for theestablishment of tissue-specific transcription profiles.Chromatin immunoprecipitation (ChIP) is a method to investigate theassociation of proteins to specific genomic loci. In this study, I have establishedtwo protocols for ChIP analyses of Xenopus laevis embryos: the In Situ ChIP andthe Douncer ChIP. In addition, I have generated several antibodies incollaboration with Dr. Elisabeth Kremmer (GSF Munchen) for ChIP analyses,which were directed against the muscle determination factor MyoD and theWnt/β-catenin signaling components Lef/Tcf transcription factors Lef1 and Tcf1.While optimizing of the ChIP protocols, I have analyzed successfullythe binding of various transcription factors, chromatin remodeling enzymes andhistone modifications on genomic loci of key developmental regulators. With theIn Situ ChIP, I have shown that the serum response factor SRF interactspredominantly with the actively transcribed myoD gene. Together with other data,this result helps to define a specific role of SRF protein in the stable maintenanceof myoD transcription, which is essential for proper muscle differentiation.With the Douncer ChIP protocol, a time course study has beenperformed in order to understand, when and which histone modification marksappear during muscle cell determination and differentiation on the myoD locus.The temporal and spatial distribution of the analyzed histone modification markswas correlated for the most part with the expected patterns. Furthermore, I havedemonstrated that direct binding of the chromatin remodeler CHD4/Mi2-β to the5' part of the sip1 gene in gastrula stage embryos. This interaction represents acrucial regulatory module, which determines the position along theanimal-vegetal axis of the embryo, where the border between the mesodermaland neuroectodermal germ layer will be formed. These examples represent on ofthe very few successful ChIP applications for the endogenous proteins in youngXenopus embryos, and I hope that my protocols will turn out useful for futureinvestigations of regulatory interactions in this vertebrate model organism." @default.
• W113377609 created "2016-06-24" @default.
• W113377609 creator A5004260267 @default.
• W113377609 date "2007-07-25" @default.
• W113377609 modified "2023-09-28" @default.
• W113377609 title "Analysis of Developmental Epistasis by Chromatin Immunoprecipitation in Xenopus laevis" @default.
• W113377609 cites W1199670722 @default.
• W113377609 cites W13033426 @default.
• W113377609 cites W1481564818 @default.
• W113377609 cites W1487395029 @default.
• W113377609 cites W1507030297 @default.
• W113377609 cites W1526135982 @default.
• W113377609 cites W1529745075 @default.
• W113377609 cites W1594700207 @default.
• W113377609 cites W1601558529 @default.
• W113377609 cites W1635724305 @default.
• W113377609 cites W167480922 @default.
• W113377609 cites W1722042447 @default.
• W113377609 cites W1767966520 @default.
• W113377609 cites W1792088282 @default.
• W113377609 cites W1826524642 @default.
• W113377609 cites W1879408402 @default.
• W113377609 cites W1938764043 @default.
• W113377609 cites W196082105 @default.
• W113377609 cites W1963736084 @default.
• W113377609 cites W1965169361 @default.
• W113377609 cites W1966517214 @default.
• W113377609 cites W1967364632 @default.
• W113377609 cites W1968877222 @default.
• W113377609 cites W1968926070 @default.
• W113377609 cites W1968931043 @default.
• W113377609 cites W1970450923 @default.
• W113377609 cites W1970709734 @default.
• W113377609 cites W1971246528 @default.
• W113377609 cites W1972215915 @default.
• W113377609 cites W1972813594 @default.
• W113377609 cites W1973756239 @default.
• W113377609 cites W1974706344 @default.
• W113377609 cites W1974832763 @default.
• W113377609 cites W1975154868 @default.
• W113377609 cites W1976443651 @default.
• W113377609 cites W1977130610 @default.
• W113377609 cites W1977302271 @default.
• W113377609 cites W1977868902 @default.
• W113377609 cites W1978287692 @default.
• W113377609 cites W1978661875 @default.
• W113377609 cites W1979124756 @default.
• W113377609 cites W1980489315 @default.
• W113377609 cites W1981417999 @default.
• W113377609 cites W1981766282 @default.
• W113377609 cites W1982035434 @default.
• W113377609 cites W1982159610 @default.
• W113377609 cites W1986773422 @default.
• W113377609 cites W1986925729 @default.
• W113377609 cites W1987086781 @default.
• W113377609 cites W1989340932 @default.
• W113377609 cites W1989721036 @default.
• W113377609 cites W1990454882 @default.
• W113377609 cites W1990586926 @default.
• W113377609 cites W1990938412 @default.
• W113377609 cites W1996683105 @default.
• W113377609 cites W1999144014 @default.
• W113377609 cites W1999155341 @default.
• W113377609 cites W2000642611 @default.
• W113377609 cites W2001191970 @default.
• W113377609 cites W2001519383 @default.
• W113377609 cites W2004553881 @default.
• W113377609 cites W2006067171 @default.
• W113377609 cites W2006935736 @default.
• W113377609 cites W2007012438 @default.
• W113377609 cites W2008509160 @default.
• W113377609 cites W2009726317 @default.
• W113377609 cites W2010041789 @default.
• W113377609 cites W2010206624 @default.
• W113377609 cites W2010390759 @default.
• W113377609 cites W2010707235 @default.
• W113377609 cites W2011286091 @default.
• W113377609 cites W2013571070 @default.
• W113377609 cites W2016603998 @default.
• W113377609 cites W2019002796 @default.
• W113377609 cites W2022138535 @default.
• W113377609 cites W2022186577 @default.
• W113377609 cites W2023246415 @default.
• W113377609 cites W2024462893 @default.
• W113377609 cites W2024584416 @default.
• W113377609 cites W2025607180 @default.
• W113377609 cites W2026290399 @default.
• W113377609 cites W2026882865 @default.
• W113377609 cites W2026898742 @default.
• W113377609 cites W2027919037 @default.
• W113377609 cites W2029903254 @default.
• W113377609 cites W2030498876 @default.
• W113377609 cites W2030523067 @default.
• W113377609 cites W2030527399 @default.
• W113377609 cites W2034191509 @default.
• W113377609 cites W2034312179 @default.
• W113377609 cites W2034932730 @default.
• W113377609 cites W2035642248 @default.
• W113377609 cites W2036347938 @default.
• W113377609 cites W2036386246 @default.

• next