Matches in SemOpenAlex for { <https://semopenalex.org/work/W1134014766> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W1134014766 abstract "Extract: The function of a gene can be genetically investigated at the DNA (gene), RNA or protein levels. Homologous recombination or gene targeting has been widely used in mice whose embryonic stem cells can be manipulated to generate mutant offspring. However, it cannot be directly applied to human genetics or primary cells. In addition, it is difficult to generate mice with mutations in multiple genes that are closely linked. Moreover, knockout mice may have cell populations that are able to compensate for the loss of gene function during embryonic development. At the RNA level, anti-sense RNA has been used with limited success. More recently, RNA interference (RNAi) has been employed to knock down gene expression in a number of organisms. Although promising, RNAi has met with various levels of success, especially if the target protein is stable, and it may influence the expression of unrelated genes through unknown mechanisms. At the protein level, one common approach to studying receptor function is through the use of blocking antibodies. However, antibodies have limitations ranging from target specificity to toxicity and in vivo efficacy. The Intrakine and Intrabody system is another method of receptor inhibition in which a cytokine or single chain antibody specific to a receptor is fused to an endoplasmic reticulum (ER) retention signal to sequester the target receptor to the ER. While the Intrakine does reduce receptor function in the target cell, it has been shown to be secreted and to stimulate receptor signaling in surrounding cells. In addition, specific targeting of proteins for degradation is being rapidly developed to investigate protein functions." @default.
- W1134014766 created "2016-06-24" @default.
- W1134014766 creator A5078959938 @default.
- W1134014766 creator A5091229755 @default.
- W1134014766 date "2003-12-01" @default.
- W1134014766 modified "2023-09-23" @default.
- W1134014766 title "Receptor-ligand genetics: Inactivation of receptors with a modified viral protein." @default.
- W1134014766 cites W1589070815 @default.
- W1134014766 cites W1986384364 @default.
- W1134014766 cites W2008879260 @default.
- W1134014766 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4415600" @default.
- W1134014766 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20705033" @default.
- W1134014766 hasPublicationYear "2003" @default.
- W1134014766 type Work @default.
- W1134014766 sameAs 1134014766 @default.
- W1134014766 citedByCount "0" @default.
- W1134014766 crossrefType "journal-article" @default.
- W1134014766 hasAuthorship W1134014766A5078959938 @default.
- W1134014766 hasAuthorship W1134014766A5091229755 @default.
- W1134014766 hasConcept C104317684 @default.
- W1134014766 hasConcept C111829913 @default.
- W1134014766 hasConcept C143065580 @default.
- W1134014766 hasConcept C153911025 @default.
- W1134014766 hasConcept C166703698 @default.
- W1134014766 hasConcept C170493617 @default.
- W1134014766 hasConcept C17971392 @default.
- W1134014766 hasConcept C54355233 @default.
- W1134014766 hasConcept C67705224 @default.
- W1134014766 hasConcept C77957584 @default.
- W1134014766 hasConcept C86803240 @default.
- W1134014766 hasConcept C95444343 @default.
- W1134014766 hasConceptScore W1134014766C104317684 @default.
- W1134014766 hasConceptScore W1134014766C111829913 @default.
- W1134014766 hasConceptScore W1134014766C143065580 @default.
- W1134014766 hasConceptScore W1134014766C153911025 @default.
- W1134014766 hasConceptScore W1134014766C166703698 @default.
- W1134014766 hasConceptScore W1134014766C170493617 @default.
- W1134014766 hasConceptScore W1134014766C17971392 @default.
- W1134014766 hasConceptScore W1134014766C54355233 @default.
- W1134014766 hasConceptScore W1134014766C67705224 @default.
- W1134014766 hasConceptScore W1134014766C77957584 @default.
- W1134014766 hasConceptScore W1134014766C86803240 @default.
- W1134014766 hasConceptScore W1134014766C95444343 @default.
- W1134014766 hasLocation W11340147661 @default.
- W1134014766 hasOpenAccess W1134014766 @default.
- W1134014766 hasPrimaryLocation W11340147661 @default.
- W1134014766 hasRelatedWork W2002009217 @default.
- W1134014766 hasRelatedWork W2030337024 @default.
- W1134014766 hasRelatedWork W2036379669 @default.
- W1134014766 hasRelatedWork W2088909687 @default.
- W1134014766 hasRelatedWork W2095869940 @default.
- W1134014766 hasRelatedWork W2144621903 @default.
- W1134014766 hasRelatedWork W2187286372 @default.
- W1134014766 hasRelatedWork W2385756224 @default.
- W1134014766 hasRelatedWork W2396078929 @default.
- W1134014766 hasRelatedWork W2949459510 @default.
- W1134014766 hasRelatedWork W2996373122 @default.
- W1134014766 hasRelatedWork W3011049299 @default.
- W1134014766 hasRelatedWork W3021224081 @default.
- W1134014766 hasRelatedWork W311084123 @default.
- W1134014766 hasRelatedWork W3186338187 @default.
- W1134014766 hasRelatedWork W85149036 @default.
- W1134014766 hasRelatedWork W2114545450 @default.
- W1134014766 hasRelatedWork W2160087659 @default.
- W1134014766 hasRelatedWork W2228229983 @default.
- W1134014766 hasRelatedWork W2594997458 @default.
- W1134014766 isParatext "false" @default.
- W1134014766 isRetracted "false" @default.
- W1134014766 magId "1134014766" @default.
- W1134014766 workType "article" @default.