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- W1134974088 abstract "Fructokinase activity is measured by estimating the appearance of the specific product, fructose-l-P. This is difficult to do in crude tissue preparations, if the contribution of the hexokinases to the phosphorylation of fructose (fructose-6-P) is not nullified. The assay utilizes two properties of hexokinases, which differentiate them from fructokinase: (1) hexokinase activity is more labile to [H+] than is fructokinase, and (2) hexokinase activity is markedly inhibited by N-acetyl-D-glucosamine, while fructokinase is relatively unaffected. The crude tissue extract may be prepared in a number of different buffer solutions. Since half the assay volume is the tissue extract as enzyme source, the tissue extract buffer contributes significantly to the final concentrations of ions and buffers used. The procedure for initial crude tissue preparation is described. The usual fructokinase assays do not directly measure the specific product fructose 1-phosphate, and appear to be accurate for fructokinase activity only when the enzyme is in a semipurified state. In a crude homogenate or in a crude 105,000 g supernatant, any fructokinase assay is inaccurate owing to the activity of nonspecific hexokinases, which may contribute 30% of the total fructose phosphorylating activity depending on physiological factors such as diet. The assay for fructokinase described in the chapter appears to overcome this problem." @default.
- W1134974088 created "2016-06-24" @default.
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- W1134974088 date "1975-01-01" @default.
- W1134974088 modified "2023-10-16" @default.
- W1134974088 title "[13] Estimation of Fructokinase (ketohexokinase) in crude tissue preparations" @default.
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- W1134974088 doi "https://doi.org/10.1016/s0076-6879(75)41015-1" @default.
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