Matches in SemOpenAlex for { <https://semopenalex.org/work/W1138496589> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W1138496589 endingPage "101" @default.
- W1138496589 startingPage "98" @default.
- W1138496589 abstract "This chapter describes the enzymic synthesis of 2-keto-3-deoxygluconate 6-phosphate using 6-phosphogluconate dehydratase. 6-Phosphogluconic dehydratase is an ethylenediaminetetraacetic acid (EDTA)-sensitive enzyme, found in the extracts of glucose-grown Pseudomonas putida, which converts 6-phosphogluconate to 2-keto-3-deoxygluconate 6-phosphate (KDPG). The KDPG aldolase-pyruvate ketimine in the extracts of the organism is reductively trapped, quantitatively inactivating the aldolase while leaving adequate dehydrase activity, which then is used to convert relatively inexpensive 6-phosphogluconate to KDPG. KDPG is then isolated as lithium salt with a good degree of purity. Glucose 6-phosphate is assayed spectrophotometrically using nicotinamide adenine dinucleotide (NAD) and glucose-6-phosphate dehydrogenase. 6-Phosphogluconate is assayed using nicotinamide adenine dinucleotide phosphate (NADP) and 6-phosphogluconate dehydrogenase. 6-Phosphogluconate dehydratase activity is determined spectrophotometrically using a system coupled to KDPG adolase, lactic dehydrogenase, and nicotinamide adenine dinucleotide dehydrogenase (NADH). The progress of the reaction is estimated by assaying perchloric acid-treated samples of KDPG. After 120 min, no further product formation is evident. No detectable 6-phosphogluconate, pyruvate, or glyceraldehyde 3-phosphate are demonstrated in the preparation by appropriate enzymic assays." @default.
- W1138496589 created "2016-06-24" @default.
- W1138496589 creator A5018288904 @default.
- W1138496589 creator A5024152497 @default.
- W1138496589 date "1982-01-01" @default.
- W1138496589 modified "2023-09-25" @default.
- W1138496589 title "[16] Enzymic synthesis of 2-keto-3-deoxygluconate 6-phosphate using 6-phosphogluconate dehydratase" @default.
- W1138496589 cites W2153435210 @default.
- W1138496589 cites W53814132 @default.
- W1138496589 cites W58358310 @default.
- W1138496589 doi "https://doi.org/10.1016/s0076-6879(82)89018-6" @default.
- W1138496589 hasPublicationYear "1982" @default.
- W1138496589 type Work @default.
- W1138496589 sameAs 1138496589 @default.
- W1138496589 citedByCount "11" @default.
- W1138496589 countsByYear W11384965892015 @default.
- W1138496589 countsByYear W11384965892020 @default.
- W1138496589 countsByYear W11384965892022 @default.
- W1138496589 crossrefType "book-chapter" @default.
- W1138496589 hasAuthorship W1138496589A5018288904 @default.
- W1138496589 hasAuthorship W1138496589A5024152497 @default.
- W1138496589 hasConcept C181199279 @default.
- W1138496589 hasConcept C185592680 @default.
- W1138496589 hasConcept C197957613 @default.
- W1138496589 hasConcept C2776317432 @default.
- W1138496589 hasConcept C2776418696 @default.
- W1138496589 hasConcept C2776901170 @default.
- W1138496589 hasConcept C2777989768 @default.
- W1138496589 hasConcept C2780196906 @default.
- W1138496589 hasConcept C2780866112 @default.
- W1138496589 hasConcept C2781374117 @default.
- W1138496589 hasConcept C28165981 @default.
- W1138496589 hasConcept C38485361 @default.
- W1138496589 hasConcept C55493867 @default.
- W1138496589 hasConcept C75520062 @default.
- W1138496589 hasConceptScore W1138496589C181199279 @default.
- W1138496589 hasConceptScore W1138496589C185592680 @default.
- W1138496589 hasConceptScore W1138496589C197957613 @default.
- W1138496589 hasConceptScore W1138496589C2776317432 @default.
- W1138496589 hasConceptScore W1138496589C2776418696 @default.
- W1138496589 hasConceptScore W1138496589C2776901170 @default.
- W1138496589 hasConceptScore W1138496589C2777989768 @default.
- W1138496589 hasConceptScore W1138496589C2780196906 @default.
- W1138496589 hasConceptScore W1138496589C2780866112 @default.
- W1138496589 hasConceptScore W1138496589C2781374117 @default.
- W1138496589 hasConceptScore W1138496589C28165981 @default.
- W1138496589 hasConceptScore W1138496589C38485361 @default.
- W1138496589 hasConceptScore W1138496589C55493867 @default.
- W1138496589 hasConceptScore W1138496589C75520062 @default.
- W1138496589 hasLocation W11384965891 @default.
- W1138496589 hasOpenAccess W1138496589 @default.
- W1138496589 hasPrimaryLocation W11384965891 @default.
- W1138496589 hasRelatedWork W1138496589 @default.
- W1138496589 hasRelatedWork W1583305934 @default.
- W1138496589 hasRelatedWork W1585891632 @default.
- W1138496589 hasRelatedWork W2006973434 @default.
- W1138496589 hasRelatedWork W2010411628 @default.
- W1138496589 hasRelatedWork W2088916855 @default.
- W1138496589 hasRelatedWork W2137996948 @default.
- W1138496589 hasRelatedWork W2972189026 @default.
- W1138496589 hasRelatedWork W3027141049 @default.
- W1138496589 hasRelatedWork W3149614979 @default.
- W1138496589 isParatext "false" @default.
- W1138496589 isRetracted "false" @default.
- W1138496589 magId "1138496589" @default.
- W1138496589 workType "book-chapter" @default.