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- W1139395712 abstract "The virus are captured using newly established magnetic beads which are coated by anti-H1N1 antibodies.Using this method,the virus was concentrated.A pair of primers was designed in subtype H5 AIV HA gene conservative region.Meanwhile,the standard plasmid containing partial sequence of subtype H5 AIV HA gene was constructed as a template,a SYBR Green Ⅰ real time RT-PCR was established.The results showed magnetic beads could absorb four HA units of AIV(H5N1) and five HA units of AIV(H1N1),but did not bind with AIV(H9N2).Depending on the enrichment of subtype N1 AIV,the RRT-PCR for detecting AIV H5 subtype showed the sensitivity was 4.6 copies/μL,while it was linear with 5-log dynamic rang.The specificity assay showed it is of high specificity,with no cross reaction to NDV,H1 and H9 AIV.So this method,magnetic bead capture system and SYBR Green Ⅰ real time RT-PCR could be applicabe to detection of AIV(H5N1)." @default.
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- W1139395712 date "2009-01-01" @default.
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- W1139395712 title "Development of magnetic bead capture system and SYBR Green I real time RT-PCR technique for detection of avian influenza A (H5N1) virus." @default.
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