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- W1141279192 abstract "This chapter discusses the NAD glycohydrolases from rat liver nuclei. The same assay mixture and procedure as used for chromatin NAD glycohydrolase are used except that 5 mM nicotinamide and 5 mM AMP are included in the mixture, in order to inhibit poly(ADPribose) synthetase and phosphodiesterase activities. The nuclei, which are precipitated through a 2.2 M sucrose layer, are washed twice with 0.25 M sucrose containing 3.3 mM CaCl2, and gently suspended in twice the packed volume of glycerol. The nuclear envelope fraction is prepared from the washed nuclei according to the method of Monneron, except that the step involving a linear gradient of 20%-50% sucrose is replaced by a discontinuous gradient of 30% and 45% sucrose containing 0.5 M MgCl2 and 50 mM Tris-HCl (pH 7.5). Centrifugation is carried out at 180,000 g for 2 h. Fractions of 0.5 ml are collected. The sharp milky-white band at the interface between the 30% and 45% sucrose layers is used as the nuclear envelope fraction. The properties of the enzyme are very similar to those of a microsomal enzyme." @default.
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- W1141279192 date "1980-01-01" @default.
- W1141279192 modified "2023-10-17" @default.
- W1141279192 title "[23] NAD glycohydrolases from rat liver nuclei" @default.
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- W1141279192 doi "https://doi.org/10.1016/0076-6879(80)66453-2" @default.
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