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- W1141542829 abstract "This chapter discusses the synthesis and effects of intermediates in purine breakdown. Xanthine is quantitatively degraded to formiminoglycine by extracts of lyophilized cells of Clostridium cylindrosporum. The enzymatic degradation of 4-ureido-5-imidazolecarboxylic acid requires either Mn++ or Fe++. This intermediate can, therefore, be formed quantitatively from xanthine by unpurified cell extracts incubated with xanthine in the presence of ethylenediaminetetraacetic acid. 4-ureido-5-imidazolecarboxylic acid may be synthesized by treating 4-amino-5-imidazolecarboxylic acid with potassium cyanide. A solution containing 7 millimoles of 4-amino-5-imidazolecarboxylic acid, 14 millimoles of potassium cyanide, and 14 millimoles of hydrochloric acid in 420 ml of water is stored at room temperature overnight. 4-amino-5-imidazolecarboxylic acid is not degraded enzymatically in media of pH above 8.0. 4-aminoimidazole accumulates briefly as the main diazotizable amine incubated at pH 7 containing xanthine and extracts of C. cylindrosporum. It is found that 4-aminoimidazole is quantitatively converted to formiminoglycine by extracts of C. cylindrosporum at pH 7.0." @default.
- W1141542829 created "2016-06-24" @default.
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- W1141542829 date "1963-01-01" @default.
- W1141542829 modified "2023-09-23" @default.
- W1141542829 title "[97] Intermedites in purine breakdown" @default.
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- W1141542829 doi "https://doi.org/10.1016/0076-6879(63)06237-6" @default.
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