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• W1148066314 abstract "This chapter describes the methods for isolation of nucleoli from various cell types and for purification of chromatin from isolated nucleoli. The nucleolus is known to be the site of ribosomal RNA (rRNA) synthesis and ribosome assembly. Association of histones and nonhistone proteins with the nucleolar DNA provides an opportunity to study the regulatory function of these proteins in a DNA-protein complex, designated as chromatin that is actively synthesizing one species of RNA. The chapter focuses on the intactness of the macromolecular structure of the nucleolus to retain in vivo function in isolated nucleoli. Isolation of nucleoli consists of three steps: (1) isolation of nuclei from whole homogenate, (2) disruption of nuclei, and (3) purification of nucleoli from the disintegrated nuclear material. Combined with advanced procedures for purification of eukaryotic RNA polymerases from various sources and with the improved techniques of analysis of histone and nonhistone proteins, isolated nucleoli and nucleolar chromatin constitute a good system for the study of gene regulation in eukaryotic cells." @default.
• W1148066314 created "2016-06-24" @default.
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• W1148066314 date "1978-01-01" @default.
• W1148066314 modified "2023-09-27" @default.
• W1148066314 title "Chapter 13 Isolation and Purification of Nucleoli and Nucleolar Chromatin from Mammalian Cells" @default.
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• W1148066314 doi "https://doi.org/10.1016/s0091-679x(08)61142-5" @default.
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