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- W115005242 abstract "2454 Objectives The aim of this prospective study was to label and perform quality control procedures of 188Re labeled Tin colloid for the treatment of Refractory Inflammatory Arthritis. Methods HCl (0.1N,0.4ml)was added in 12 mg of SnCl2.2H2O.The solution divided into five equal parts in separate vials and sealed.Freshly eluted 2-4ml 188ReO4- (perrhenate)with saline from 188W/188Re-generator was added in each of the SnCl2.2H2O containing vials.Solution boiled for 30,60,90,120min and one sample was kept at room temp.188Re-Sn microparticles were neutralized by adding 0.2M Na3PO4 buffer (PH 8.0) and centrifuged for 20 min at 3000 rpm. The pellets of 188Re-Sn resuspended in 0.9% saline.The radioactivity in supernatant and precipitate was measured.Stability of 188Re-Sn microparticles was assessed in normal saline solution. Radiolabeling efficiency was determined by ITLC-SG/normal saline plates. The batches of 188Re-Sn microparticles kept at room temp for 24 & 48h after labeling and stability were again evaluated by ITLC-SG/normal saline.All samples of microparticles were visualized under scanning electron microscope and the size were determined. Results It was observed that as soon as HCl was added in SnCl2, the small spherical colloidal particles of mean size 0.76 µm were formed. As heating progressed to 100 C,the colloidal particles clustered and formed spherical microparticles.Mean size of microparticles prepared at 30,60,90,120min were 2.6, 4.3,7.3 & 7.8µm respectively. After centrifugation the formulation (90 min heating),98-99% microparticles were of mean size 7.3µm.Mean radiolabelling efficiency of microparticles formed at room temp, after boiling 30,60, 90,120min were 28.7%,46%,92.4%, 96.2% & 97% respectively. Conclusions The particle size distribution and labeling efficiencies were altered by increasing the heating time(60/90min),however, these properties remain unaltered even after heating for 120 min, hence ideal time for heating should be 90 min" @default.
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- W115005242 date "2011-05-01" @default.
- W115005242 modified "2023-09-23" @default.
- W115005242 title "Preparation and quality control of 188Re labeled Tin colloid for the treatment of refractory inflammatory arthritis" @default.
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