Matches in SemOpenAlex for { <https://semopenalex.org/work/W1150395152> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W1150395152 endingPage "4215" @default.
- W1150395152 startingPage "4212" @default.
- W1150395152 abstract "Objective:To construct recombinant adenovirus carrying IGF2 imprinting system and to investigate the expression of the reporter gene EGFP of the recombinant adenovirus in tumor cells and normal cells. Methods: A IGF2 imprinting system was constructed via inserting promoter H19,CCCTC binding factor (CTCF), enhancer of IGF2 and reporter gene EGFP into shuttle plasmid pDC-312. Then the recombinant plasmid and Ad5 skeleton were co-transfected into HEK293 cells for packaging. Recombinant adenovirus Ad-H19-CTCF-enhancer-EGFP (short for Ad-EGFP) was detected by PCR and the infection titer was monitored. The expression of the reporter gene EGFP of Ad-EGFP was tested after infecting IGF2 loss of impriting (LOI)cancer cell line HRT-18 and IGF2 maintenance of imprinting (MOI)cancer cell line MCF-7and normal cell line GES-1. Results: The IGF2 imprinting system was cloned into the adenovirus successfully. Viral titer was about 5.5×l09pfu/ml. Fluorescence microscope observation demonstrated that the IGF2 impriting system works in IGF2 LOI cancer cells but not in IGF2 MOI cells. Conclusions:The constructed recombinant adenovirus containing IGF2 impriting system provides a foundation for investigating the application of the system in tumor targeted therapy." @default.
- W1150395152 created "2016-06-24" @default.
- W1150395152 creator A5003849340 @default.
- W1150395152 creator A5026146274 @default.
- W1150395152 creator A5037323203 @default.
- W1150395152 creator A5062026165 @default.
- W1150395152 creator A5069921645 @default.
- W1150395152 creator A5072684195 @default.
- W1150395152 creator A5086883564 @default.
- W1150395152 creator A5089420213 @default.
- W1150395152 date "2009-01-01" @default.
- W1150395152 modified "2023-09-22" @default.
- W1150395152 title "Construction and identification of recombinant adenovirus carrying IGF2 imprinting system." @default.
- W1150395152 hasPublicationYear "2009" @default.
- W1150395152 type Work @default.
- W1150395152 sameAs 1150395152 @default.
- W1150395152 citedByCount "0" @default.
- W1150395152 crossrefType "journal-article" @default.
- W1150395152 hasAuthorship W1150395152A5003849340 @default.
- W1150395152 hasAuthorship W1150395152A5026146274 @default.
- W1150395152 hasAuthorship W1150395152A5037323203 @default.
- W1150395152 hasAuthorship W1150395152A5062026165 @default.
- W1150395152 hasAuthorship W1150395152A5069921645 @default.
- W1150395152 hasAuthorship W1150395152A5072684195 @default.
- W1150395152 hasAuthorship W1150395152A5086883564 @default.
- W1150395152 hasAuthorship W1150395152A5089420213 @default.
- W1150395152 hasConcept C104317684 @default.
- W1150395152 hasConcept C111936080 @default.
- W1150395152 hasConcept C142613039 @default.
- W1150395152 hasConcept C150194340 @default.
- W1150395152 hasConcept C153911025 @default.
- W1150395152 hasConcept C159047783 @default.
- W1150395152 hasConcept C161733203 @default.
- W1150395152 hasConcept C174510640 @default.
- W1150395152 hasConcept C22744801 @default.
- W1150395152 hasConcept C2778760011 @default.
- W1150395152 hasConcept C32470452 @default.
- W1150395152 hasConcept C40767141 @default.
- W1150395152 hasConcept C54009773 @default.
- W1150395152 hasConcept C54355233 @default.
- W1150395152 hasConcept C81885089 @default.
- W1150395152 hasConcept C86803240 @default.
- W1150395152 hasConceptScore W1150395152C104317684 @default.
- W1150395152 hasConceptScore W1150395152C111936080 @default.
- W1150395152 hasConceptScore W1150395152C142613039 @default.
- W1150395152 hasConceptScore W1150395152C150194340 @default.
- W1150395152 hasConceptScore W1150395152C153911025 @default.
- W1150395152 hasConceptScore W1150395152C159047783 @default.
- W1150395152 hasConceptScore W1150395152C161733203 @default.
- W1150395152 hasConceptScore W1150395152C174510640 @default.
- W1150395152 hasConceptScore W1150395152C22744801 @default.
- W1150395152 hasConceptScore W1150395152C2778760011 @default.
- W1150395152 hasConceptScore W1150395152C32470452 @default.
- W1150395152 hasConceptScore W1150395152C40767141 @default.
- W1150395152 hasConceptScore W1150395152C54009773 @default.
- W1150395152 hasConceptScore W1150395152C54355233 @default.
- W1150395152 hasConceptScore W1150395152C81885089 @default.
- W1150395152 hasConceptScore W1150395152C86803240 @default.
- W1150395152 hasIssue "22" @default.
- W1150395152 hasOpenAccess W1150395152 @default.
- W1150395152 hasRelatedWork W2347796310 @default.
- W1150395152 hasRelatedWork W2348466846 @default.
- W1150395152 hasRelatedWork W2348904362 @default.
- W1150395152 hasRelatedWork W2349415525 @default.
- W1150395152 hasRelatedWork W2351825124 @default.
- W1150395152 hasRelatedWork W2356563149 @default.
- W1150395152 hasRelatedWork W2359841857 @default.
- W1150395152 hasRelatedWork W2360372382 @default.
- W1150395152 hasRelatedWork W2363173893 @default.
- W1150395152 hasRelatedWork W2365719068 @default.
- W1150395152 hasRelatedWork W2371408049 @default.
- W1150395152 hasRelatedWork W2372227229 @default.
- W1150395152 hasRelatedWork W2372786050 @default.
- W1150395152 hasRelatedWork W2374745218 @default.
- W1150395152 hasRelatedWork W2376466341 @default.
- W1150395152 hasRelatedWork W2377849370 @default.
- W1150395152 hasRelatedWork W2379327071 @default.
- W1150395152 hasRelatedWork W2380731165 @default.
- W1150395152 hasRelatedWork W2381101320 @default.
- W1150395152 hasRelatedWork W2406318196 @default.
- W1150395152 hasVolume "9" @default.
- W1150395152 isParatext "false" @default.
- W1150395152 isRetracted "false" @default.
- W1150395152 magId "1150395152" @default.
- W1150395152 workType "article" @default.