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- W1152959805 abstract "This chapter describes the basic protocols for the production and purification of IL-1 from the P388D1 macrophage cell line, along with assays used to quantitate IL-1 activity. A basic protocol for generating goat antibodies to IL-1 is also explained. Interleukin I (IL-1) 1 is a polypeptide of 12,000 to 17,000 molecular weight that functions as a major communication signal between the macrophage and other cell types that are involved in immune and inflammatory responses. This polypeptide is produced by peripheral blood, peritoneal, alveolar, and placental macrophages. In addition, a number of IL-l-producing glioma and keratinocyte tumor cell lines are described. Murine IL-1 was initially purified to homogeneity as a result of (1) the use of the P388D1 murine macrophage cell line and (2) the use of a superinduction protocol for producing relatively high levels of IL- 1. The chapter further discusses the purification of murine interleukin 1." @default.
- W1152959805 created "2016-06-24" @default.
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- W1152959805 date "1985-01-01" @default.
- W1152959805 modified "2023-09-25" @default.
- W1152959805 title "[37] Murine interleukin 1" @default.
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- W1152959805 doi "https://doi.org/10.1016/s0076-6879(85)16039-8" @default.
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