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- W115456075 abstract "The analysis of DNA-methylation on a genome-wide scale by next-generation sequencing techniques is an invaluable tool towards the understanding of the epigenetic basis of cellular differentiation. Methylated DNA immunoprecipitation (MeDIP) is an immunocapturing method using an antibody targeting 5-methylcytidine (5mC) and thereby enriching methylated DNA. MeDIP combined with next-generation sequencing (MeDIP-seq) provides a powerful tool for the analysis of genome-wide DNA-methylation profiles. Here, we describe a protocol for the preparation of MeDIP samples suitable for next-generation sequencing on a Genome Analyser (Illumina)." @default.
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- W115456075 date "2012-01-01" @default.
- W115456075 modified "2023-10-16" @default.
- W115456075 title "Analysis of the Methylome of Human Embryonic Stem Cells Employing Methylated DNA Immunoprecipitation Coupled to Next-Generation Sequencing" @default.
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- W115456075 doi "https://doi.org/10.1007/978-1-61779-794-1_19" @default.
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