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- W1155077666 abstract "Author(s): Anderson, Timothy David | Advisor(s): Clubb, Robert T | Abstract: Dwindling petroleum reserves have intensified efforts to develop renewable biofuels from highly abundant resources like lignocellulosic plant biomass. This dissertation focuses on the use of synthetic biology as well as a variety of microbiology and molecular biology approaches to develop microorganisms that efficiently degrade plant biomass. A microbial surface display system was constructed that enabled the successful covalent attachment of cellulases and multi-cellulase cellulosome complexes to the peptidoglycan of the Bacillus subtilis cell wall. Proteins containing the appropriate cell wall sorting signal were attached by co-expressing the sortase transpeptidase from Bacillus anthracis. Cellulosome complexes containing three cellulase enzymes were displayed. By co-expressing different components complexes could either be assembled ex vivo or spontaneously. The cells released significant amounts of sugars when exposed to regenerated amorphous cellulose (RAC), dilute acid pretreated corn stover, as well as untreated corn stover, switchgrass and straw. Expansion of the cellulosomal complexes to display six cellulase enzymes led to dramatically increased lignocellulose solubilization with the cellulolytic capacity of the cells approaching values of released sugars measured for commercially available cellulase cocktails. Importantly, the cells displaying these self-assembled cellulosomes were able to grow robustly on lignocellulosic substrates as the sole source of carbon, suggesting that the strains developed may be useful in the construction of a consolidated bioprocessing (CBP) microorganism that can produce biofuels and other biocommodities directly from lignocellulose." @default.
- W1155077666 created "2016-06-24" @default.
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- W1155077666 date "2013-01-01" @default.
- W1155077666 modified "2023-09-27" @default.
- W1155077666 title "Engineering the Surface of Bacillus subtilis to Degrade Lignocellulose for Biofuel Production" @default.
- W1155077666 hasPublicationYear "2013" @default.
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