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• W1157062228 abstract "This chapter presents the preparation of the enzymes of sterol biosynthesis from liver, the enzymatic and/or chemical synthesis of all the intermediates of squalene biosynthesis from mevalonate up to and including farnesyl pyrophosphate. All the methods described in this chapter are those developed and used in the laboratories. The chapter describes chemical syntheses of substratcs of sterol biosynthesis: (1) syntheses suitable for the preparation of 1-14C - and 2-14C-labeled mevalonolactone; (2) syntheses of unlabeled and of 3’,4-13C2-1abeled mevalonolactone; and (3) synthesis of R(—)mevalonolactone (natural isomer). It also describes the synthesis of 5-D2- and 4-14C-labeled mevalonolaetone; synthesis of mevalonates labeled stereospecifieally at C-4 with hydrogen isotopes; preparation of mevalonates labeled stereospecifieally at C-2 with hydrogen isotopes; and synthesis of methyl and ethyl farnesoates; procedure suitable for labeling with 14C at C-2. The synthesis of isopentenyl pyrophosphate and prenyl pyrophosphates are described. All the assays used so far for the enzymatic synthesis of the prenyl pyrophosphates from l4C-labeled mevalonate, or from 14C-labeled isopentenyl pyrophosphate, depend on their instability to acid and on the resulting liberation of the primary and tertiary l4C-labeled alcohols, extractable from the incubations either with ether or with light petroleum." @default.
• W1157062228 created "2016-06-24" @default.
• W1157062228 creator A5056203575 @default.
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• W1157062228 date "1969-01-01" @default.
• W1157062228 modified "2023-10-18" @default.
• W1157062228 title "[11] Chemical syntheses of substrates of sterol biosynthesis" @default.
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• W1157062228 doi "https://doi.org/10.1016/s0076-6879(69)15013-2" @default.
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