FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W115741766> ?p ?o ?g. }

• W115741766 endingPage "284" @default.
• W115741766 startingPage "243" @default.
• W115741766 abstract "This chapter discusses the theories and applications of various nucleic acid-based approaches such as gene probes, microarrays, conventional and real-time polymerase chain reaction (PCR), whole genome amplification, sequencing, comparative genomics, and metagenomics. Gene probes are single-stranded DNA, and can be used to identify a particular nucleic acid sequence within an environmental sample through colony hybridization, Southern and Northern hybridization (used for DNA and RNA respectively), Fluorescent in situ hybridization (FISH), microarrays, and phyloarrays. PCR is extremely sensitive and is able to detect a single target molecule in some cases. The use of PCR based techniques—such as reverse transcriptase–PCR (RT–PCR), introduced integrated cell culture-PCR (ICC-PCR), arbitrarily primed-PCR (AP-PCR), and Real-Time PCR has enabled the researchers to detect specific nucleic acid sequences in isolates as well in environmental samples. Recombinant DNA technology or DNA cloning has been widely used to examine the genetics of bacterial populations. Metagenomics is another nucleic acid-based approach that allows analysis of multiple genes and screening of large environmental fragments for functional activities. Several computer software sequence analysis programs and tools—such as Basic Local Alignment Sequence Tool (BLAST) or Clustal W—are available for sequences searches, and comparative genomics in such cases allows the comparison of subsets of genes or whole genomes of interest. Restriction fragment length polymorphism (RFLP) and its modifications such as amplified fragment length polymorphisms (AFLP) and terminal-restriction fragment length polymorphism (T-RFLP) can characterize or differentiate the whole genomes or specific PCR products from bacterial isolates. Denaturing gradient gel electrophoresis (DGGE) or temperature gradient gel electrophoresis (TGGE) has been employed to assess the microbial diversity. Plasmid analysis has been used to detect specific bacteria or genes such as in Bacillus anthracis." @default.
• W115741766 created "2016-06-24" @default.
• W115741766 creator A5020104676 @default.
• W115741766 creator A5065238239 @default.
• W115741766 creator A5084579733 @default.
• W115741766 date "2009-01-01" @default.
• W115741766 modified "2023-09-30" @default.
• W115741766 title "Nucleic Acid–Based Methods of Analysis" @default.
• W115741766 cites W1543289866 @default.
• W115741766 cites W1551721102 @default.
• W115741766 cites W1553864302 @default.
• W115741766 cites W1558365920 @default.
• W115741766 cites W1588533534 @default.
• W115741766 cites W1589053113 @default.
• W115741766 cites W1669459870 @default.
• W115741766 cites W1963563104 @default.
• W115741766 cites W1966249879 @default.
• W115741766 cites W1966637572 @default.
• W115741766 cites W1971403296 @default.
• W115741766 cites W1980898928 @default.
• W115741766 cites W1987177651 @default.
• W115741766 cites W1994714652 @default.
• W115741766 cites W1995854433 @default.
• W115741766 cites W2001620437 @default.
• W115741766 cites W2013404388 @default.
• W115741766 cites W2016842763 @default.
• W115741766 cites W2016880237 @default.
• W115741766 cites W2016992246 @default.
• W115741766 cites W2017112652 @default.
• W115741766 cites W2019410656 @default.
• W115741766 cites W2022227920 @default.
• W115741766 cites W2022462377 @default.
• W115741766 cites W2023829651 @default.
• W115741766 cites W2025334511 @default.
• W115741766 cites W2026903678 @default.
• W115741766 cites W2029357992 @default.
• W115741766 cites W2032579724 @default.
• W115741766 cites W2034090035 @default.
• W115741766 cites W2035524115 @default.
• W115741766 cites W2043806842 @default.
• W115741766 cites W2045770689 @default.
• W115741766 cites W2046762542 @default.
• W115741766 cites W2060199965 @default.
• W115741766 cites W2070721758 @default.
• W115741766 cites W2075716829 @default.
• W115741766 cites W2076981350 @default.
• W115741766 cites W2077199974 @default.
• W115741766 cites W2086408159 @default.
• W115741766 cites W2094613397 @default.
• W115741766 cites W2096625211 @default.
• W115741766 cites W2100057072 @default.
• W115741766 cites W2105861220 @default.
• W115741766 cites W2107913200 @default.
• W115741766 cites W2111858111 @default.
• W115741766 cites W2114849808 @default.
• W115741766 cites W2117765216 @default.
• W115741766 cites W2125800973 @default.
• W115741766 cites W2126132619 @default.
• W115741766 cites W2143258005 @default.
• W115741766 cites W2145166062 @default.
• W115741766 cites W2147783737 @default.
• W115741766 cites W2148995598 @default.
• W115741766 cites W2152453780 @default.
• W115741766 cites W2156896172 @default.
• W115741766 cites W2158427719 @default.
• W115741766 cites W2158714788 @default.
• W115741766 cites W2159895759 @default.
• W115741766 cites W2160211110 @default.
• W115741766 cites W2161618076 @default.
• W115741766 cites W2161863335 @default.
• W115741766 cites W2164095252 @default.
• W115741766 cites W2164231744 @default.
• W115741766 cites W2169267759 @default.
• W115741766 cites W2170404450 @default.
• W115741766 cites W2316109371 @default.
• W115741766 cites W310567034 @default.
• W115741766 cites W4231607407 @default.
• W115741766 cites W4246548745 @default.
• W115741766 cites W4248176371 @default.
• W115741766 doi "https://doi.org/10.1016/b978-0-12-370519-8.00013-4" @default.
• W115741766 hasPublicationYear "2009" @default.
• W115741766 type Work @default.
• W115741766 sameAs 115741766 @default.
• W115741766 citedByCount "5" @default.
• W115741766 countsByYear W1157417662013 @default.
• W115741766 countsByYear W1157417662014 @default.
• W115741766 countsByYear W1157417662023 @default.
• W115741766 crossrefType "book-chapter" @default.
• W115741766 hasAuthorship W115741766A5020104676 @default.
• W115741766 hasAuthorship W115741766A5065238239 @default.
• W115741766 hasAuthorship W115741766A5084579733 @default.
• W115741766 hasConcept C104317684 @default.
• W115741766 hasConcept C141231307 @default.
• W115741766 hasConcept C144024400 @default.
• W115741766 hasConcept C149923435 @default.
• W115741766 hasConcept C150194340 @default.
• W115741766 hasConcept C15151743 @default.
• W115741766 hasConcept C153523973 @default.

• next