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- W1158440326 abstract "This chapter discusses genetic tools and presents the detail of the steps in the generation, isolation, genetic analysis, and interpretation of mutations affecting development of D.discoideum. Dictyostelium discoideum contains a single gene coding for myosin heavy chain. While it might be expected to be an essential gene for growth, mutations affecting certain specific portions of myosin might not disrupt its functions so drastically as to preclude growth. The availability of monoclonal antibodies to seven different portions of the myosin heavy chain prompted to screen for mutations affecting one or another of these portions. Two mutations are recovered that result in loss of the epitope recognized by monoclonal antibody My4 without affecting reactivity to the other four myosin-specific monoclonal antibodies. The mutations are recessive, noncomplementing, and map to linkage group II (LGII). Molecular analyses show that the mutations cause alterations in the carboxy-terminal region of the heavy chain of myosin. Strains carrying either of these mutations aggregate, however fail to complete development." @default.
- W1158440326 created "2016-06-24" @default.
- W1158440326 creator A5014612742 @default.
- W1158440326 date "1987-01-01" @default.
- W1158440326 modified "2023-10-14" @default.
- W1158440326 title "Chapter 3 Genetic Tools for Dictyostelium discoideum" @default.
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- W1158440326 doi "https://doi.org/10.1016/s0091-679x(08)61636-2" @default.
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