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- W1163480260 abstract "The chytridiomycosis is a significant emerging disease of amphibians in recent years which seriuosly does harm to the wild and breeding population.To ascertain the condition of the Batrachochytrium dendrobatidis infection history in the population of toad,the medicinal economic animal,and enhance the prevention for this disease,32 toad specimen collected from Sichwan keeping in a museum were assayed by Taqman-MGB fluorescence probe quantitative polymerase chain reaction to detect Batrachochytrium dendrobatidis.Besides,the products of QPCR were cloned and sequenced to identify the origin of the pathogen by sequence alignment and phylogenetic analysis.Finally we got the standard curve:Y=-3.0X+32.39 and the related coefficient:R2=0.999 6. Total 12 positive samples reported the detection rate as 37.5%.Meanwhile the phylogenetic analysis indicated that a certain extent differentiation of the chytrid fungi in our country exists.One kind of the tested strain showed highly genetic relationship with the strains from the North America,South America and Europe.Another one is obviously different from the strains found in the other areas of the world with special characteristics.It suggest that the prevention and treatment for chytridiomycosis of toad should be more targeted and should not copy foreign experience aimlessly." @default.
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- W1163480260 date "2011-01-01" @default.
- W1163480260 modified "2023-09-23" @default.
- W1163480260 title "Genetic differentiation of the pathogen of Batrachochytrium dendrobatidis in toads." @default.
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