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- W116804524 abstract "This chapter discusses the ligation of genomic DNA into bacteriophage arms and packaging to form library. The prepared partial Mbo I-digested genomic DNA is ligated into the prepared bacteriophage arms. The best ratio of genomic DNA fragments to cloning vector for optimal library production is determined empirically in a series of trial reactions. The remainder of the genomic DNA fragments are ligated to the vector at the optimal ratio, and the recombinant phage are packaged in vitro . The packaged phage are then used to infect an appropriate E. coli host to generate the library. The time required to follow this method is two days. On the first day, it takes one hour, and on the second day, it takes 2–4 hours. The chapter lists the reagents that are needed for the purpose, such as T4 DNA ligase, TMG buffer, and packaging extract." @default.
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- W116804524 date "1986-01-01" @default.
- W116804524 modified "2023-09-28" @default.
- W116804524 title "Ligation of Genomic DNA into Bacteriophage Arms and Packaging to Form Library" @default.
- W116804524 doi "https://doi.org/10.1016/b978-0-444-01082-7.50052-7" @default.
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