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- W1169238404 abstract "Objective To explore the role of mouse dermis-derived mesenchymal stem cells (mdMSC) on skin repair. Methods mdMSC and human dermal fibroblasts were isolated and identified. Human dermal fibroblasts were cultured alone or cocultured with mdMSC in Transwell chambers with the density ratio of human dermal fibroblasts to mdMSC being 2/5, 1/1, and 2/1. On day 4 and 8 of culture, the expression levels of hydroxyproline and transforming growth factor-beta (TGF-beta) 1 were measured in the supernatant of monoculture and coculture by alkaline hydrolysis and ELISA respectively. Results The level of hydro-xyproline was significantly higher in the supernatants of coculture system with a density ratio of 2/5 and 1/1 than that in monoculture supernatants of human dermal fibroblasts on day 8 (both P 0.05). Conclusion In vitro coculture with mdMSC can increase the production of hydroxyproline and TGF-betal by fibroblasts, which may be a mechanism underlying the facilitation of skin repair by mdMSC.Key words: Dennis; Mesenchymal stem cell transplantation; Fibroblasts" @default.
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- W1169238404 date "2009-07-15" @default.
- W1169238404 modified "2023-09-24" @default.
- W1169238404 title "Effect of coculture with mouse dermis-derived mesenchymal stem cells on the secretion of collagen and expression of transforming growth factor-beta1 by human dermal fibroblasts." @default.
- W1169238404 doi "https://doi.org/10.3760/cma.j.issn.0412-4030.2009.07.018" @default.
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