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- W117130305 abstract "The authors are currently studying the effects of Zn deficiency on chromatin structure, DNA synthesis, protein synthesis and cell division in the rat. Here the authors present preliminary results of nuclear DNA measurement by flow cytometry. Propidium iodide (PI) was used as a fluorescent probe for total nuclear DNA isolated from Day 18 fetal rat brain. Previously, dams were fed a control (50 ..mu..g Zn/g diet), restricted (50 ..mu..g Zn/g diet) or a Zn deficient (0.5 ..mu..g Zn/g diet) diet. Fetuses were taken at Day 18 of gestation by casaerean section. Whole brain was quickly excised and frozen rapidly in a plastic vial which was subsequently immersed in an alcohol/dry ice bath at -80/sup 0/C. Brain was thawed at 37/sup 0/C in a citrate/DMSO/pH 7.6 buffer and dissociated by several passes through a 26 gauge needle. Nuclei were isolated and stained by incubating in 50 ..mu..g/ml PI, 0.125 ..mu..g/ml RNA-ASE, 0.1% NP-40 at 25/sup 0/C. Zn deficiency resulted in a larger coefficient of variation (CV) for the Go/Gi DNA peak in fetal brain nuclei. The DNA profile of these nuclei is suggestive of a possible block at the Go/Gi-S interphase; restricted fed and ad lib fed controls showed no suchmore » blockage.« less" @default.
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- W117130305 date "1986-03-05" @default.
- W117130305 modified "2023-09-23" @default.
- W117130305 title "Flow cytometry analysis of cell cycle stages in zinc deficient fetal rat brain" @default.
- W117130305 hasPublicationYear "1986" @default.
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