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- W1172920269 abstract "Immunofluorescence is a well-established technique for the detection of antigens in tissue sections or cell suspensions (1, 2). The technique was developed in 1941 by Albert Coons to demonstrate the presence of pneumococcal antigens in tissue (3). Since then, immunofluorescence has become a crucial tool in the diagnosis and determination of prognoses of immunologically mediated disease (4). Direct immunofluorescence (defined as the application of specific antibodies to detect specific antigens in tissue) is a sensitive and well-established technique for the detection of tissue-bound immunoglobulins, their subclasses, complement components (C3, C1q, and C4d), amyloidogenic proteins, and fibrin-fibrinogen. In the kidney, many forms of primary glomerulonephritis are characterized by deposition of immunoreactants in distinctive diagnostic patterns. The primary renal targets are the glomerulus, the proximal tubules, and the interstitium. In the heart or open fat pad biopsy specimens, direct immunofluorescence is useful to speciate the type of amyloid identified on specimens with positive Congo red staining in paraffin-embedded tissue (5). The diagnosis of antibody-mediated rejections in heart and kidney allografts is facilitated by the detection of C4d (6–8). This chapter will also emphasize the common immunofluorescence techniques used for diagnosis and interpretation with kidney and skin biopsy specimens." @default.
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- W1172920269 date "2016-01-27" @default.
- W1172920269 modified "2023-09-29" @default.
- W1172920269 title "Immunofluorescence Methods in the Diagnosis of Renal and Cardiac Diseases" @default.
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- W1172920269 doi "https://doi.org/10.1128/9781555818722.ch41" @default.
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