FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W117310306> ?p ?o ?g. }

• W117310306 endingPage "4957" @default.
• W117310306 startingPage "4947" @default.
• W117310306 abstract "Factor(s) that bind gonadotropins have been extracted from rat testis by 30% ethanol (v/v) in water and their interaction with human lutropin (hLH) and human follitropin (hFSH) have been investigated by a new assay using dextran-coated charcoal. These studies reveal that: 1. Maximal binding of gonadotropin with soluble factors was observed over a broad range of pH from 6.0 to 8.0 with a relative decline in binding at extremes of pH. The binding was independent of the ionic strength of the buffer and reached equilibrium within 5 min at 4 degrees, 27 degrees, and 37 degrees. 2. The soluble factors have marked thermostability, a point of distinction from detergent-solubilized receptors. 3. The equilibrium dissociation constant (Kd) of 125I-hFSH binding to the soluble factor was 6.0 +/- 0.58 X 10(-10) M, consistent with the values obtained from the membrane binding studies. Similarly, the Kd value for 125I-hLH to the soluble factor(s) was 3.33 +/- 0.3 X 10(-9) M, comparable to the values obtained from the membrane binding studies. Hill plots demonstrated a lack of a cooperative relationship with an apparent Hill coefficient of 1.071 for hLH and 0.909 for hFSH. Furthermore, two classes of binding sites for 125I-human choriogonadotropin (hCG) were clearly discernible by both Lineweaver-Burk and Hill plots with an equilibrium dissociation constant of 2.4 +/- 0.5 X 10(-11) M and 1.35 +/- 1.2 X 10(-9) M. The apparent Hill coefficient of interaction of 125I-hCG with the soluble factors was found to be 0.923 for high affinity and 1.09 for low affinity binding sites. 4. The binding of 125I-hLH and 125I-hFSH with respect to concentrations of soluble factor(s) was found to be a saturable process, yielding an expected 4.4-fold higher Kd for hLH (294 +/- 13.8 mug/ml) compared to hFSH (66.6 +/- 4 mug/ml). These findings are comparable with the equilibrium dissociation constants, thus confirming a 5-fold higher affinity of hFSH as compared to hLH for the soluble factors, i.e. the ratio of 3.0 X 10(-9) M to 6.0 X 10(-10) M versus the ratio of 294 mug/ml to 66.6 mug/ml. 5. The hormone specificity of the interaction has been studied by using radiolabeled hFSH, hLH, hCG, prolactin, growth hormone, and bovine serum albumin. The binding of FSH at low factor concentrations was found to be 5- to 10-fold greater than prolactin, growth hormone, and albumin. 6. The soluble factors are found in higher concentration in testis compared to liver, kidney, and blood. 7. The effect of ethanol upon solubilization of the factor(s) has been investigated. The factor(s) can be extracted with buffer or water alone. However, 10 to 25% of ethanol (v/v) facilitates the process of solubilization. The treatment with 70% ethanol (v/v) or more did not extract any factor activity from testes. The factor(s) were insoluble in petroleum ether, chloroform, absolute ethanol, methanol, or lipid solvent. 8. Finally the effect of soluble factors on classical membrane binding was investigated..." @default.
• W117310306 created "2016-06-24" @default.
• W117310306 creator A5046570285 @default.
• W117310306 creator A5059658537 @default.
• W117310306 creator A5087435698 @default.
• W117310306 creator A5091783863 @default.
• W117310306 date "1976-08-01" @default.
• W117310306 modified "2023-10-17" @default.
• W117310306 title "Gonadotropin binding factor(s). Extraction of high affinity gonadotropin binding sites from rat testis and partial characterization of their interaction with human follitropin, lutropin, and choriogonadotropin." @default.
• W117310306 cites W1495169618 @default.
• W117310306 cites W1496129614 @default.
• W117310306 cites W1497345935 @default.
• W117310306 cites W1503983718 @default.
• W117310306 cites W1505117697 @default.
• W117310306 cites W1515318096 @default.
• W117310306 cites W1519150778 @default.
• W117310306 cites W1525663151 @default.
• W117310306 cites W1532274733 @default.
• W117310306 cites W1532746392 @default.
• W117310306 cites W1552439704 @default.
• W117310306 cites W1563193952 @default.
• W117310306 cites W1588148271 @default.
• W117310306 cites W1597663270 @default.
• W117310306 cites W1603557977 @default.
• W117310306 cites W162964340 @default.
• W117310306 cites W1775749144 @default.
• W117310306 cites W1893651774 @default.
• W117310306 cites W19158468 @default.
• W117310306 cites W1925015362 @default.
• W117310306 cites W1972420511 @default.
• W117310306 cites W1979957843 @default.
• W117310306 cites W1982783652 @default.
• W117310306 cites W1983649348 @default.
• W117310306 cites W1989090188 @default.
• W117310306 cites W2002934776 @default.
• W117310306 cites W2009262099 @default.
• W117310306 cites W2010184839 @default.
• W117310306 cites W2016242080 @default.
• W117310306 cites W2021127650 @default.
• W117310306 cites W2052790041 @default.
• W117310306 cites W2054746030 @default.
• W117310306 cites W2072740613 @default.
• W117310306 cites W2073975368 @default.
• W117310306 cites W2076933139 @default.
• W117310306 cites W2079570220 @default.
• W117310306 cites W2082950566 @default.
• W117310306 cites W2094074268 @default.
• W117310306 cites W2141449078 @default.
• W117310306 cites W2283761167 @default.
• W117310306 cites W2335083472 @default.
• W117310306 cites W4231152632 @default.
• W117310306 cites W4233151359 @default.
• W117310306 cites W4247598639 @default.
• W117310306 doi "https://doi.org/10.1016/s0021-9258(17)33206-4" @default.
• W117310306 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/182687" @default.
• W117310306 hasPublicationYear "1976" @default.
• W117310306 type Work @default.
• W117310306 sameAs 117310306 @default.
• W117310306 citedByCount "27" @default.
• W117310306 countsByYear W1173103062019 @default.
• W117310306 crossrefType "journal-article" @default.
• W117310306 hasAuthorship W117310306A5046570285 @default.
• W117310306 hasAuthorship W117310306A5059658537 @default.
• W117310306 hasAuthorship W117310306A5087435698 @default.
• W117310306 hasAuthorship W117310306A5091783863 @default.
• W117310306 hasBestOaLocation W1173103061 @default.
• W117310306 hasConcept C126322002 @default.
• W117310306 hasConcept C134018914 @default.
• W117310306 hasConcept C185592680 @default.
• W117310306 hasConcept C2778782382 @default.
• W117310306 hasConcept C43617362 @default.
• W117310306 hasConcept C4725764 @default.
• W117310306 hasConcept C71315377 @default.
• W117310306 hasConcept C71924100 @default.
• W117310306 hasConcept C86803240 @default.
• W117310306 hasConceptScore W117310306C126322002 @default.
• W117310306 hasConceptScore W117310306C134018914 @default.
• W117310306 hasConceptScore W117310306C185592680 @default.
• W117310306 hasConceptScore W117310306C2778782382 @default.
• W117310306 hasConceptScore W117310306C43617362 @default.
• W117310306 hasConceptScore W117310306C4725764 @default.
• W117310306 hasConceptScore W117310306C71315377 @default.
• W117310306 hasConceptScore W117310306C71924100 @default.
• W117310306 hasConceptScore W117310306C86803240 @default.
• W117310306 hasIssue "16" @default.
• W117310306 hasLocation W1173103061 @default.
• W117310306 hasOpenAccess W117310306 @default.
• W117310306 hasPrimaryLocation W1173103061 @default.
• W117310306 hasRelatedWork W1976589237 @default.
• W117310306 hasRelatedWork W2037126054 @default.
• W117310306 hasRelatedWork W2052893624 @default.
• W117310306 hasRelatedWork W2066027832 @default.
• W117310306 hasRelatedWork W2072823512 @default.
• W117310306 hasRelatedWork W2087941795 @default.
• W117310306 hasRelatedWork W2099713939 @default.
• W117310306 hasRelatedWork W2135637476 @default.
• W117310306 hasRelatedWork W2135688739 @default.
• W117310306 hasRelatedWork W2163517010 @default.

• next