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- W1174567738 abstract "Publisher Summary This chapter presents several methods for handling a limited amount of material for electron microscopy. The techniques of preliminary embedding in agar, of microcentrifugation, and of embedding in a capillary tube are most useful for free cells, such as protozoa; the various modifications of the flat, in situ embedding technique have been most widely used with cultured cells, grown and embedded on a flat surface. Most investigations with the electron microscope have dealt with tissues or suspensions of large numbers of free cells or cell organelles. If free cells or their parts are available in sufficient quantity, they may be centrifuged into a pellet and subsequently processed just as a piece of tissue. When, however, experiments have to deal with visually selected and/or manually manipulated cells, the electron microscopist is confronted with the task of keeping track of a microscopic amount of material through the prolonged processes of selection, fixation, dehydration, embedding, and sectioning. Single free cells, such as protozoa, may be embedded in a capillary tube. If more material is available, the use of a microcentrifuge is quick and efficient." @default.
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- W1174567738 date "1966-01-01" @default.
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- W1174567738 title "Chapter 11 Methods for Handling Small Numbers of Cells for Electron Microscopy" @default.
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