Matches in SemOpenAlex for { <https://semopenalex.org/work/W1176239518> ?p ?o ?g. }
Showing items 1 to 58 of
58
with 100 items per page.
- W1176239518 abstract "The Na/K pump is a heterodimeric (αβ) protein that generates Na and K gradients essential for excitability and secondary active transport. Cardiac muscle presents at least two types of Na/K pump α-subunits (α1 and α2) that combine with two β-subunits (β1 and β2), with the physiological combinations believed to be α1β1 and α2β2. In membrane preparations, different isoforms present slightly different kinetic parameters. We performed voltage-clamp experiments at physiological concentrations of Nao and Ko in rat ventricular myocytes and in Xenopus oocytes expressing several human Na/K pump isoforms. Our results indicate that the distinct voltage-dependent characteristics of the Na/K pump isoforms may be more relevant than previously thought. In particular, we demonstrate that while α1 pumps are fully functional at all voltages, α2 pump function is drastically reduced and nearly zero at resting membrane potentials (~-75 mV), but maximally-active at depolarized potentials. This observation provides a simple explanation for the physiological relevance of these pumps (~20% of total pumps in rat ventricle), acting as a reservoir for extra Na/K pumping during the long-lasting cardiac action potential, where most of the Na and Ca entry occur through ion channels. Furthermore, by measuring transient currents without Ko, we demonstrate that these effects correlate with the increased affinity of α2 pumps for Nao. Our results also help explain the beneficial effects of cardiotonic steroids by only inhibiting these reservoir pumping pool and suggest that post-translational modification of Na/K pump voltage dependent reactions maybe an important regulatory mechanism. NIH R15-NS081570-01" @default.
- W1176239518 created "2016-06-24" @default.
- W1176239518 creator A5003026525 @default.
- W1176239518 creator A5016246700 @default.
- W1176239518 creator A5042484234 @default.
- W1176239518 creator A5083568656 @default.
- W1176239518 date "2015-04-01" @default.
- W1176239518 modified "2023-09-26" @default.
- W1176239518 title "Physiological relevance of isoform‐specific voltage dependence in the Na/K pump" @default.
- W1176239518 doi "https://doi.org/10.1096/fasebj.29.1_supplement.845.32" @default.
- W1176239518 hasPublicationYear "2015" @default.
- W1176239518 type Work @default.
- W1176239518 sameAs 1176239518 @default.
- W1176239518 citedByCount "0" @default.
- W1176239518 crossrefType "journal-article" @default.
- W1176239518 hasAuthorship W1176239518A5003026525 @default.
- W1176239518 hasAuthorship W1176239518A5016246700 @default.
- W1176239518 hasAuthorship W1176239518A5042484234 @default.
- W1176239518 hasAuthorship W1176239518A5083568656 @default.
- W1176239518 hasConcept C104317684 @default.
- W1176239518 hasConcept C12554922 @default.
- W1176239518 hasConcept C158154518 @default.
- W1176239518 hasConcept C17744445 @default.
- W1176239518 hasConcept C185592680 @default.
- W1176239518 hasConcept C199539241 @default.
- W1176239518 hasConcept C53345823 @default.
- W1176239518 hasConcept C55493867 @default.
- W1176239518 hasConcept C86803240 @default.
- W1176239518 hasConceptScore W1176239518C104317684 @default.
- W1176239518 hasConceptScore W1176239518C12554922 @default.
- W1176239518 hasConceptScore W1176239518C158154518 @default.
- W1176239518 hasConceptScore W1176239518C17744445 @default.
- W1176239518 hasConceptScore W1176239518C185592680 @default.
- W1176239518 hasConceptScore W1176239518C199539241 @default.
- W1176239518 hasConceptScore W1176239518C53345823 @default.
- W1176239518 hasConceptScore W1176239518C55493867 @default.
- W1176239518 hasConceptScore W1176239518C86803240 @default.
- W1176239518 hasFunder F4320332161 @default.
- W1176239518 hasIssue "S1" @default.
- W1176239518 hasLocation W11762395181 @default.
- W1176239518 hasOpenAccess W1176239518 @default.
- W1176239518 hasPrimaryLocation W11762395181 @default.
- W1176239518 hasRelatedWork W1586527248 @default.
- W1176239518 hasRelatedWork W2016582329 @default.
- W1176239518 hasRelatedWork W2021149666 @default.
- W1176239518 hasRelatedWork W2062409570 @default.
- W1176239518 hasRelatedWork W2064506701 @default.
- W1176239518 hasRelatedWork W2095494064 @default.
- W1176239518 hasRelatedWork W2103316668 @default.
- W1176239518 hasRelatedWork W2144955858 @default.
- W1176239518 hasRelatedWork W2293128458 @default.
- W1176239518 hasRelatedWork W356665176 @default.
- W1176239518 hasRelatedWork W2186523205 @default.
- W1176239518 hasVolume "29" @default.
- W1176239518 isParatext "false" @default.
- W1176239518 isRetracted "false" @default.
- W1176239518 magId "1176239518" @default.
- W1176239518 workType "article" @default.