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- W1180491843 abstract "Samples of raw and treated sewage were collected from several treatment plants as the liquid expressed from cheesecloth swabs exposed to flowing sewage for from 24 to 48 hr. This sampling technique has been valuable for the detection of bacterial (2) and virus pathogens (3) that may occur intermittently in sewage. It was ob served that these swab expressions con tained enteroviruses and bacteria from two to four times more often than comparable catch samples (3) (4). Sev eral catch samples were also obtained. The volume of swab expression was approximately 0.1 1; that of catch samples, either 0.4 or 4 1. All samples were concentrated on Dowex 1 resin (3), eluted, treated with antibiotics and diethyl ether to reduce bacterial and fungal contaminants, and stored in a dry-ice chest. The eluates were added in 0.5-ml amounts to mono layers of monkey kidney epithelium tissue that had been grown several days in 4-oz bottles. The inoculated tissue cultures were rinsed with 199H solution one-half hour later and over laid with a nutrient agar containing neutral red (5). They were incubated at 33 ?C and examined daily for plaque formation until the tissue degenerated. Virus presence was confirmed by propa gating a salt diffusate from the plaques in tube cultures of monkey kidney epithelium tissue. Viruses were identi fied serologically by neutralization tests. A few eluates were also tested for plaque formation in bottle cul tures of FL amnion cells (6). Virus densities were estimated from the number of plaques formed, cor rected for the total volume of sample. The results are expressed as number of plaque-forming units (PFU) in 100 ml of swab expression. A sample cal culation follows:" @default.
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- W1180491843 date "1960-01-01" @default.
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- W1180491843 title "Density of enteroviruses in sewage." @default.
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