FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W118118902> ?p ?o ?g. }

• W118118902 endingPage "201" @default.
• W118118902 startingPage "190" @default.
• W118118902 abstract "Penicillin-binding proteins (PBPs) are the natural targets of beta-lactam antibiotics and mutations in pbp1a, pbp2b, and pbp2x genes, which encode PBPs, are responsible for resistance to beta-lactams in Streptococcus pneumoniae. In the present study, we intended to determine how often the common mutation patterns occurred within the pbp1a, pbp2b, and pbp2x PBP gene regions and evaluate the PBP genotype mutations which were associated with penicillin resistance in several penicillin-susceptible and - resistant S.pneumoniae isolates in Mersin, Turkey. A total of 62 S.pneumoniae strains isolated from different clinical specimens (32 nasopharyngeal swab, 16 sputum, 3 blood, 3 wound, 2 cerebrospinal fluids and one of each urine, abscess, bronchoalveolar lavage, conjunctival swab, tracheal aspirate, middle ear effusion) were included in the study. Penicillin susceptibilities of the isolates were searched by disc diffusion and E-test methods, and 23 of them were identified as susceptible, 31 were intermediate susceptible, and eight were resistant to penicillin. A rapid DNA extraction procedure was performed for the isolation of nucleic acids from the strains. Distribution of PBP gene mutations in pbp1a, pbp2b, and pbp2x gene regions related to penicillin resistance was determined by using a wild-type specific polymerase chain reaction (PCR) based technique. PBP gene alterations of those isolates were also evaluated in relation to penicillin susceptibility and resistance patterns. Twenty two (95.7%) of 23 penicillin-susceptible S.pneumoniae isolates exhibited no mutation in the three PBP genes (pbp1a, pbp2x, and pbp 2b), while 1 (4.3%) of these harbored mutations in all of the three PBP genes. The penicillin-intermediate susceptible S.pneumoniae isolates exhibited various combinations of mutations. One (3.2%) of 31 penicillin-intermediate susceptible isolates exhibited no mutation in the three PBP genes, while 22 (71%) of them yielded mutations in all of the three PBP genes. The remaining 8 (25.8%) isolates harbored mutations for dual PBP genes (in five strains pbp1a and pbp2b; in two strains pbp2x and pbp2b; in one strain pbp1a and pbp2x). Seven (87.5%) out of eight penicillin-resistant S.pneumoniae isolates (MIC ≥ 2 µg/ml) revealed mutations in all of the three PBP genes and the other penicillin-resistant isolates exhibited no mutation in the PBP genes. The present study supplied important data on the frequency of different patterns of mutations occurring at various regions of PBP genes related to penicillin resistance in S.pneumoniae isolates in our restricted region. The results supported the notion that penicillin resistance in S.pneumoniae was mainly attributed to alterations in pbp1a, pbp2x, and pbp2b gene regions and wild-type sequence specific PCR could be applied to characterize genotypic background of penicillin resistance in S.pneumoniae strains." @default.
• W118118902 created "2016-06-24" @default.
• W118118902 creator A5012630433 @default.
• W118118902 creator A5023374544 @default.
• W118118902 creator A5056191283 @default.
• W118118902 creator A5076087549 @default.
• W118118902 creator A5079616087 @default.
• W118118902 creator A5091311915 @default.
• W118118902 date "2012-04-01" @default.
• W118118902 modified "2023-09-23" @default.
• W118118902 title "[Evaluation of penicillin-binding protein genotypes in penicillin susceptible and resistant Streptococcus pneumoniae isolates]." @default.
• W118118902 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22639308" @default.
• W118118902 hasPublicationYear "2012" @default.
• W118118902 type Work @default.
• W118118902 sameAs 118118902 @default.
• W118118902 citedByCount "1" @default.
• W118118902 countsByYear W1181189022023 @default.
• W118118902 crossrefType "journal-article" @default.
• W118118902 hasAuthorship W118118902A5012630433 @default.
• W118118902 hasAuthorship W118118902A5023374544 @default.
• W118118902 hasAuthorship W118118902A5056191283 @default.
• W118118902 hasAuthorship W118118902A5076087549 @default.
• W118118902 hasAuthorship W118118902A5079616087 @default.
• W118118902 hasAuthorship W118118902A5091311915 @default.
• W118118902 hasConcept C104317684 @default.
• W118118902 hasConcept C135763542 @default.
• W118118902 hasConcept C174802600 @default.
• W118118902 hasConcept C2776653525 @default.
• W118118902 hasConcept C2781253189 @default.
• W118118902 hasConcept C501593827 @default.
• W118118902 hasConcept C54355233 @default.
• W118118902 hasConcept C86803240 @default.
• W118118902 hasConcept C89423630 @default.
• W118118902 hasConceptScore W118118902C104317684 @default.
• W118118902 hasConceptScore W118118902C135763542 @default.
• W118118902 hasConceptScore W118118902C174802600 @default.
• W118118902 hasConceptScore W118118902C2776653525 @default.
• W118118902 hasConceptScore W118118902C2781253189 @default.
• W118118902 hasConceptScore W118118902C501593827 @default.
• W118118902 hasConceptScore W118118902C54355233 @default.
• W118118902 hasConceptScore W118118902C86803240 @default.
• W118118902 hasConceptScore W118118902C89423630 @default.
• W118118902 hasIssue "2" @default.
• W118118902 hasLocation W1181189021 @default.
• W118118902 hasOpenAccess W118118902 @default.
• W118118902 hasPrimaryLocation W1181189021 @default.
• W118118902 hasRelatedWork W1737038474 @default.
• W118118902 hasRelatedWork W1932616695 @default.
• W118118902 hasRelatedWork W2026224862 @default.
• W118118902 hasRelatedWork W2063452348 @default.
• W118118902 hasRelatedWork W2104240326 @default.
• W118118902 hasRelatedWork W2137110239 @default.
• W118118902 hasRelatedWork W2149759952 @default.
• W118118902 hasRelatedWork W2152585568 @default.
• W118118902 hasRelatedWork W2157977667 @default.
• W118118902 hasRelatedWork W2321522859 @default.
• W118118902 hasVolume "46" @default.
• W118118902 isParatext "false" @default.
• W118118902 isRetracted "false" @default.
• W118118902 magId "118118902" @default.
• W118118902 workType "article" @default.