FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W118156936> ?p ?o ?g. }

• W118156936 endingPage "1088" @default.
• W118156936 startingPage "1083" @default.
• W118156936 abstract "Abstract Blood donations are routinely screened by multiple serologic assays for antigens/antibodies associated with infection by blood-borne viruses, including hepatitis B virus (HBV), hepatitis C virus (HCV), human immunodeficiency viruses (HIV-1 and HIV-2), and human T-cell lymphotropic virus (HTLV-I and HTLV-II). A direct detection of these viruses would be more effective for the prevention of transfusion- transmitted infections than the indirect measurement of the variable host immune response to these agents. Because the polymerase chain reaction (PCR) for viral gene amplification offers the most sensitive and direct means of detecting viruses in blood, we have developed a nonisotopic PCR procedure for the detection of HBV, chosen as a prototype. The problems, common to previously described PCR methods, of nucleic acid extraction and inhibition of the PCR by plasma proteins were overcome by isolation of HBV from plasma by means of 450-microns polystyrene beads covalently coated with monoclonal antibody to the Pre- S1 region of the viral envelope protein. Detergent lysis and proteinase K digestion of the immunocaptured virions isolated from plasma released the HBV DNA. A modified PCR-amplification protocol, incorporating digoxigenin-labeled dUTP in the amplified gene products followed by hybridization with a specific biotinylated oligonucleotide probe bound to streptavidin-coated 2.8-microns magnetic beads, allowed flow cytometric analyses of HBV-specific PCR products by means of antibodies to digoxigenin labeled with fluorescein isothiocyanate. The endpoint serial dilutions of pedigreed human plasma samples containing chimpanzee infectious dose (CID50) of 10(7) for adw and CID50 of 10(7.5) for the ayw subtypes were compared in repeated testing of PCR products by our immunoreactive bead (PCR-IRB) assay. HBV DNA was consistently detected in a 5 x 10(-10) dilution of each sample. In testing 20 coded specimens of blood donors, with or without serologic markers of HBV infection, the PCR-IRB was specific and more sensitive than the PCR analyses by slot blot hybridization with radioactive probe. The PCR-IRB assay can be adapted for simultaneous detection of multiple blood-borne viruses by an automated flow cytometric analysis system." @default.
• W118156936 created "2016-06-24" @default.
• W118156936 creator A5010270454 @default.
• W118156936 creator A5019004713 @default.
• W118156936 creator A5024477661 @default.
• W118156936 creator A5079324585 @default.
• W118156936 creator A5089269266 @default.
• W118156936 date "1993-02-15" @default.
• W118156936 modified "2023-10-16" @default.
• W118156936 title "Detection of hepatitis B virus in plasma using flow cytometric analyses of polymerase chain reaction-amplified DNA incorporating digoxigenin-11- dUTP" @default.
• W118156936 cites W1479837533 @default.
• W118156936 cites W1547932352 @default.
• W118156936 cites W1971957622 @default.
• W118156936 cites W1972462008 @default.
• W118156936 cites W1973376153 @default.
• W118156936 cites W1983764580 @default.
• W118156936 cites W2032118018 @default.
• W118156936 cites W2048962369 @default.
• W118156936 cites W2050717506 @default.
• W118156936 cites W2115496818 @default.
• W118156936 cites W2167983049 @default.
• W118156936 cites W2406887109 @default.
• W118156936 doi "https://doi.org/10.1182/blood.v81.4.1083.1083" @default.
• W118156936 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8427989" @default.
• W118156936 hasPublicationYear "1993" @default.
• W118156936 type Work @default.
• W118156936 sameAs 118156936 @default.
• W118156936 citedByCount "26" @default.
• W118156936 countsByYear W1181569362013 @default.
• W118156936 crossrefType "journal-article" @default.
• W118156936 hasAuthorship W118156936A5010270454 @default.
• W118156936 hasAuthorship W118156936A5019004713 @default.
• W118156936 hasAuthorship W118156936A5024477661 @default.
• W118156936 hasAuthorship W118156936A5079324585 @default.
• W118156936 hasAuthorship W118156936A5089269266 @default.
• W118156936 hasBestOaLocation W1181569361 @default.
• W118156936 hasConcept C104317684 @default.
• W118156936 hasConcept C150194340 @default.
• W118156936 hasConcept C153911025 @default.
• W118156936 hasConcept C159047783 @default.
• W118156936 hasConcept C159654299 @default.
• W118156936 hasConcept C203014093 @default.
• W118156936 hasConcept C2522874641 @default.
• W118156936 hasConcept C2776552150 @default.
• W118156936 hasConcept C2780593183 @default.
• W118156936 hasConcept C49105822 @default.
• W118156936 hasConcept C55493867 @default.
• W118156936 hasConcept C81439078 @default.
• W118156936 hasConcept C86803240 @default.
• W118156936 hasConceptScore W118156936C104317684 @default.
• W118156936 hasConceptScore W118156936C150194340 @default.
• W118156936 hasConceptScore W118156936C153911025 @default.
• W118156936 hasConceptScore W118156936C159047783 @default.
• W118156936 hasConceptScore W118156936C159654299 @default.
• W118156936 hasConceptScore W118156936C203014093 @default.
• W118156936 hasConceptScore W118156936C2522874641 @default.
• W118156936 hasConceptScore W118156936C2776552150 @default.
• W118156936 hasConceptScore W118156936C2780593183 @default.
• W118156936 hasConceptScore W118156936C49105822 @default.
• W118156936 hasConceptScore W118156936C55493867 @default.
• W118156936 hasConceptScore W118156936C81439078 @default.
• W118156936 hasConceptScore W118156936C86803240 @default.
• W118156936 hasIssue "4" @default.
• W118156936 hasLocation W1181569361 @default.
• W118156936 hasOpenAccess W118156936 @default.
• W118156936 hasPrimaryLocation W1181569361 @default.
• W118156936 hasRelatedWork W2054848430 @default.
• W118156936 hasRelatedWork W2056420846 @default.
• W118156936 hasRelatedWork W2070179873 @default.
• W118156936 hasRelatedWork W2118787634 @default.
• W118156936 hasRelatedWork W2139983200 @default.
• W118156936 hasRelatedWork W2140679976 @default.
• W118156936 hasRelatedWork W2165013436 @default.
• W118156936 hasRelatedWork W2328038080 @default.
• W118156936 hasRelatedWork W3140282071 @default.
• W118156936 hasRelatedWork W3143876889 @default.
• W118156936 hasVolume "81" @default.
• W118156936 isParatext "false" @default.
• W118156936 isRetracted "false" @default.
• W118156936 magId "118156936" @default.
• W118156936 workType "article" @default.